京海黄鸡IGF-Ⅱ基因SNPs及其与生产性能关系研究  

SNPs of IGF-Ⅱ Gene and Its Correlations to Production Performance in Jinghai Yellow Chicken

作　　者：杨凤萍[1] 李其松[2] 戴国俊[2] 谢恺舟[2] 施会强 王金玉[2] 

机构地区：[1]扬州大学生物科学与技术学院,扬州225009 [2]扬州大学动物科学与技术学院,扬州225009 [3]海门京海集团有限公司,海门226100

出　　处：《畜牧兽医学报》2008年第11期1470-1475,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省高新技术资助(BG2005307)

摘　　要：以京海黄鸡为材料,采用PCR-SSCP技术测定IGF-Ⅱ基因3个SNPs位点,同时分析它们对不同周龄体重及产蛋性状的遗传效应。结果显示,3个位点分别发生C→G、G→C、G→A的单碱基突变,各检测到3种基因型,P1位点偏离Hardy-Weinberg平衡状态(P<0.05)。最小二乘方差分析表明,AA与BB基因型个体300日龄产蛋数存在显著差异(P<0.05),A为增效等位基因,B为减效等位基因;CD基因型个体12周龄体质量显著高于CC型(P<0.05);EE、EF基因型个体300日龄平均蛋质量显著高于FF型(P<0.05),E为增效等位基因,F为减效等位基因。联合基因型AA/CC/EF平均蛋质量显著高于AB/CD/EE与AB/CD/EF(P<0.05);AB/CD/EF基因型个体300日龄体质量显著高于AA/CC/EF、BB/DD/EE型(P<0.05)。Three SNP sites of IGF -Ⅱ gene in Jinghai Yellow chicken was detected by PCR-SSCP method, its genetic effects on production performance were also analyzed. And the results showed that the mutations of C-→G,G→C,G→A in P1 ,P2,P3 sites were identified, respectively. There were three genotypes in each site, and three genotypes in P1 site deviated the Hardy-Wein- berg equilibrium（P^0.05）. The Least Square Means analysis showed the difference of the egg number in 300 days between AA and BB genotypes were significant（P〈0.05）, and A was an en- hancing allele, B was a leaky one; CD has higher 12-week-weight than CC（P〈0.05） ; EE and EF have higher average-egg-weight than FF（P〈0.05）, and E was an enhancing allele, F was a leaky one. The com-genotypes AA/CC/EF has higher average-egg-weight than AB/CD/EE and AB/ CD/EF（P〈0.05） ; AB/CD/EF has higher 300-day-weight than AA/CC/EF and BB/DD/EE（P〈 0.05）.

关 键 词：京海黄鸡 IGF-Ⅱ SNPS 组合基因型 生产性能 

分 类 号：S813.2[农业科学—畜牧学] Q527.3[农业科学—畜牧兽医]