猪Krox20基因组织表达规律及其在脂肪细胞中表达规律的研究  

Study on the Expression Rule of Krox20 Gene in Tissue and Adipocyte

作　　者：甄鑫[1] 阮井玲[1] 周佳勃[2] 刘娣 

机构地区：[1]东北农业大学动物科技学院,哈尔滨150030 [2]东北农业大学生命科学学院,哈尔滨150030 [3]黑龙江省农业科学院,哈尔滨150086

出　　处：《畜牧兽医学报》2008年第11期1476-1481,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30270950);教育部留学回国人员科研启动基金

摘　　要：采用半定量RT-PCR方法对1月龄和6月龄大白猪,1月龄野猪Krox20基因的表达规律进行研究,并且还检测猪脂肪细胞和不同月龄大白猪脂肪组织Krox20基因的表达情况。结果表明Krox20基因在大白猪和野猪的心、肝、脾、肺、肾、胃等12个组织中均有表达;各组织间表达存在差异,在心、肝、脂肪组织中高表达;1月龄大白和1月龄野猪Krox20的表达模式基本相同,不存在种属特异性;Krox20基因在不同时期的脂肪组织的表达随日龄的增加而降低,9月龄表达量最低;脂肪细胞水平的研究中,在前脂肪细胞中未检测到Krox20的表达,只有在诱导之后的5 h内,检测到Krox20的瞬时表达。The expression of Krox20 gene of Yorkshire in 1,6 months and Wild boar in 1 month were analyzed by using semi-quantitative RT-PCR method and detected in adipocyte and adipose tissue. The results showed that Krox20 gene was expressed in heart, liver, spleen, lung, kidney, stomach, large intestine, small intestine, fat, ovary, uterus, muscle; The expression level of Krox20 gene was different in the detected tissues, it was highly expressed in heart, liver, fat; The expression mode of 1 month Yorkshire and Wild boar were similar, there was little species- specificity; With the age increasing, the expression of Krox20 gene was gradually decreased in adipose tissue,it had the highest expression level at 1 month, and reached the least at 9 month; In the research of adipocyte, the expression of Krox20 gene was not detected in preadipoeyte, but detected in adipocyte after induced for 5 hours.

关 键 词：猪 Krox20基因 脂肪 表达 

分 类 号：S828.2[农业科学—畜牧学]