曲古抑菌素(Trichostatin)A对猪卵母细胞体外成熟及孤雌胚胎发育能力的影响  

Effect of Trichostatin A on in vitro Maturation of Porcine Oocyte and Development of Parthenogenetic Embryo

作　　者：刘晓[1,2] 潘登科[1] 陈扣扣[1,3] 冯冲[1,3] 张卫红[1,3] 郑茂恩[1,4] 龙川[1] 冯书堂[1] 杨博辉[2] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与利用重点开放实验室,北京100193 [2]中国农业科学院兰州畜牧与兽药研究所,兰州730050 [3]甘肃农业大学,兰州730070 [4]云南农业大学,昆明650201

出　　处：《畜牧兽医学报》2008年第11期1493-1498,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家“863”项目(2006AA02Z113);中国农业科学院科技经费项目资助

摘　　要：曲古抑菌素A(Trichostatin A,TSA)是一种组蛋白去乙酰化抑制剂,用TSA处理鼠核移植胚胎可显著提高胚胎的囊胚率。检验TSA对猪卵母细胞体外成熟以及孤雌胚胎发育的影响。在猪卵母细胞体外成熟液及胚胎培养液中添加TSA,比较不同浓度TSA对卵母细胞成熟的影响,不同浓度TSA对孤雌胚胎发育能力的影响以及TSA处理不同时间对孤雌胚胎发育能力的影响。结果发现:(1)5 nmol/L TSA处理对卵母细胞体外核成熟无显著影响,却显著提高了卵母细胞孤雌胚胎的卵裂率和囊胚率(P<0.05);(2)50 nmol/L TSA处理显著提高了孤雌胚胎的卵裂率及囊胚率(P<0.05);(3)50 nmol/L TSA处理24 h能显著提高胚胎的卵裂率及囊胚率(P<0.05,82.1%±2.6%和37.4%±3.1%)。结果表明TSA对猪卵母细胞的体外成熟及孤雌胚胎发育具有显著的促进作用。5 nmol/L的添加量对卵母细胞的体外胞质成熟具有促进作用;胚胎培养基中添加50 nmol/L TSA处理24 h能提高孤雌胚胎的发育能力。Trichostatin A （TSA） is an inhibitor of histone deacetylase. It is reported that treatment of mouse somatic cell nuclear-transferred oocytes with TSA significantly increased the blas- tocyst rate. The present study was designed to examine the effect of TSA on the maturation of porcine oocytes and development of parthenogenetic embryos in vitro. We evaluated the concen- tration of TSA to oocyte maturation, the concentration of TSA to parthenogenetic embryo, treatment duration of TSA to parthenogenetic embryo in vitro. The maturation oocytes to the metaphase Ⅱ （M Ⅱ） stage cultured with 5 nmol/L TSA was not significantly different from the TSA free treatment, meanwhile 5 nmol/L TSA treatment supported a higher cleavage and blastoeyst development rate （P〈0. 05） . The parthenogenetic embryo exposured in 50 nmol/L TSA supported a higher cleavage and blastoeyst development rate （P〈0.05）. The parthenogenetic embryo exposured in 50 nmol/L TSA for 24 h supported a higher cleavage and blastocyst development rate than the others （P〈0.05, 82.1±2.6% and 37.4 3.1% ）. The data demonstrated that treatment of porcine oocytes and parthenogenetic embryos with TSA significantly improved the in vitro blastocyst production. 5 nmol/L TSA treatment enhanced the oocytes maturation in vitro, and 50 nmol/L TSA-treatment for 24 h following oocyte activation resulted in more efficient de- velopment of parthenogenetic embryo.

关 键 词：猪 TSA 卵母细胞 体外成熟 孤雌胚胎 

分 类 号：S828.3[农业科学—畜牧学]