大豆黄酮和染料木素对体外培养奶牛乳腺上皮细胞增殖及抗氧化水平的影响  

Effect of Daidzein and Genistein on Proliferation and Antioxidation of Mammary Epithelial Cell of Dairy Cow in vitro

作　　者：刘春龙[1,2] 李忠秋[3] 张帆[1] 姜文博[1] 徐岩[1] 单安山[1] 

机构地区：[1]东北农业大学动物营养研究所,哈尔滨150030 [2]中国科学院东北地理与农业生态研究所黑土生态重点实验室,哈尔滨150081 [3]黑龙江省农业科学院畜牧研究所,哈尔滨150086

出　　处：《畜牧兽医学报》2008年第11期1517-1522,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：黑龙江省教育厅重大计划资助项目(10541z003);哈尔滨市青年科技创新人才专项基金资助项目(2006RFQYN111);国家科技支撑计划资助项目(2006BAD04A03-02)

摘　　要：通过体外细胞培养的方法探讨大豆黄酮(Daidzein,Da)和染料木素(Genistein,Ge)对奶牛乳腺上皮细胞增殖及抗氧化水平的影响。试验分空白对照组、10 ng/mL雌二醇(E2)组、不同浓度Da和Ge(1、10、100、1000ng/mL)组,培养72 h后,采用MTT法检测细胞增殖情况。结果表明,10、100 ng/mL Ge组和100、1000 ng/mL Da组同空白对照组比较可显著促进奶牛乳腺上皮细胞的增殖(P<0.05),但均显著低于E2组(P<0.05)。另取处于对数生长期的乳腺上皮细胞加入不同浓度Da和Ge(1、10、100、1000 ng/mL)继续培养24 h,检测细胞培养液中总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性、丙二醛(MDA)、一氧化氮(NO)含量。结果表明,100、1000 ng/mL Da组及1001、000 ng/mL Ge组较空白组显著提高了T-SOD活性(P<0.05),显著降低了MDA含量(P<0.05);100、1000 ng/mL Da组及10、100、1000 ng/mL Ge组显著提高了NO含量(P<0.05);1000ng/mL Da组及100、1000 ng/mL Ge组显著提高了GSH-PX活性(P<0.05)。可见,Da和Ge在一定浓度范围内均有促进奶牛乳腺上皮细胞增殖及抗氧化水平提高的作用。Effect of thelial cell of dairy Daidzein and Genistein on the proliferation and antioxidation of mammary epicow were determined in vitro. The experiments were assigned into 9 groups （blank,1, 10,100,1 000 ng/mL Daidzein and Genistein）. According to MMT,the proliferation ex periment results showed tha ly improved dairy cow mam t 10,100 ng/mL Genistein mary epithelial cell prolif and 100,1 000 ng/mL Daidzein significanteration （P〈0.05） ng/mL E2 group （P〈0. 05） after 72 h cultured. In the other experiment but all weaker than 10 the mammary epithelial cell during the logarithm growing period were collected after 24 h cultured to measured the T- SOD,GSH-PX activity,MDA,NO content. Results showed that 100,1 000 ng/mL Daidzein and 100,1 000 ng/mL Genistein significantly increased the T-SOD activites and decreased MDA con- centration （P〈0. 05）. 100,1 000 ng/mL Daidzein and 10,100, 1 000 ng/mL Genistein signifi- cantly increased NO concentration （P〈0.05）. 1 000 ng/mL Daidzein and 100, 1 000 ng/mL Genistein significantly increased the GSH-PX activites （P〈0.05）. It was proved that proper levels of Daidzein and Genistein can advance the proliferation and antioxidation of mammary epithelial cell of dairy cow.

关 键 词：大豆黄酮 染料木素 奶牛乳腺上皮细胞 增殖 抗氧化 

分 类 号：S823.91[农业科学—畜牧学] Q253[农业科学—畜牧兽医]