两种组胺受体拮抗剂对肺动脉高压肉鸡右心功能的影响  

Effects of Two Histamine Receptor Antagonists on Right Ventricle's Function in Broiler Chickens with Pulmonary Hypertension

作　　者：欧德渊[1,2] 乔健[1] 刘文菊[1] 田兴贵[2] 高铭宇[1] 李静[1] 

机构地区：[1]中国农业大学动物医学院,北京100193 [2]贵州大学动物科学学院,贵阳550025

出　　处：《畜牧兽医学报》2008年第11期1588-1593,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30371063);贵州省优秀人才省长资金[黔省专合字(2005)349号]

摘　　要：探讨组胺H，受体拮抗剂扑尔敏和H。受体拮抗剂西咪替丁对低温诱发肺动脉高压肉鸡右心功能的影响，由此反证内源性组胺在低温诱发肉鸡肺动脉高压形成过程中的作用。采用160只17日龄AA肉鸡随机分为4组，每组40只。（1）常温组：在常温条件下饲养（22～23℃），每天注射生理盐水2次；（2）低温组：低温环境中饲养（9～11℃），每天注射生理盐水2次；（3）扑尔敏组：与低温组相同饲养，每天注射扑尔敏2次；（4）西眯替丁组：与低温组相同饲养，每天注射西眯替丁2次。分别于低温处理后1周（24日龄）、2周（31日龄）、3周（38日龄）、4周（45日龄）从每组中各随机抽取7只，利用右心导管法测定右心室内压和右心室压最大变化速率，计算腹水心脏指数（AHI）和右心衰竭率。剖杀后用常规阿尔新蓝法对心脏中肥大细胞（MC）染色，免疫组化方法显示组胺。结果：（1）低温处理后使肉鸡肥大细胞和组胺阳性细胞数量显著降低，但2种组胺受体拮抗剂对肥大细胞数量及组胺阳性细胞数无明显影响；（2）扑尔敏组肉鸡右心内压相对稳定，45日龄时右心室舒张压显著低于低温组（P〈0．05）。西咪替丁组肉鸡右心收缩压和舒张压处于较低水平，且在31和38日龄时显著低于低温组（P〈0．05）；（3）扑尔敏组右心内压最大变化速率相对稳定，31日龄时显著低于低温组（P〈0．05），而45日龄时显著高于低温组（P〈0．05）。西咪替丁组右心内压最大变化速率处于较低水平；（4）45日龄时，扑尔敏组AHI显著低于低温组（P〈0．05）；（5）扑尔敏组显著（P〈0．05）降低了右心肥大指数和右心衰竭发生率，西咪替丁组仅显著（P〈0．05）降低了右心衰竭率。以上结果表明，内源性组胺可能通过H3受体介导肉鸡右心肥大和衰竭，从而促进肺动脉高压综合征的�The aim of the study was to evaluate the effect of histamine receptor antagonist on right ventricle＇s function in broiler chickens with pulmonary hypertension induced by low ambient temperature. One hundred and sixty male 17-day-old broilers were divided randomly into four groups. There are forty birds in each group. （1） Normal temperature group （NT） ： The birds were reared at 22-23 ,Cand normal saline was given twice by intramuscular injection each day. （2） Low ambitent temperature group （LT）： The birds were reared at 9-11℃and normal saline was given twice each day. （3） Chlorphenamine maleate group in low ambient temperature （CMLT） ： The birds were reared as LT, and chlorphenamine maleate was given twice each day. （4） Cimetidine group in low ambient temperature （CLT） ： The birds were reared as LT, and cimetidine was given twice each day. At 24, 31, 38, 45 days of age, 7 birds were randomly taken from each group for measurement of right ventricular pressure （RVP） using right cardiac catheter. Mast cells （MC） was stained with Alicience blue. Histamine was shown using immunohistochemistry. The ascites heart index （AHI） were accounted. The results were as follows. （1） The number of mast cells and histamine positive cells were decreased by low ambient temperature, but it was no affected by chlorphenamine maleate and cimetidine. （2） The RVP of CMLT group was maintained at same levels, and the RVP of CLT group was kept at low levels. （3） The dp/dt of CMLT group was significantly （P〈0. 05） different with that of LT group. （4） AHI of LT group was significantly higher than that of NT and CMLT group （P〈0. 05）. （5） The ratio of right ventricular hypertrophy and failure in CMLT group were lower than those in LT group. It was concluded that internal histamine can promote the development of pulmonary hypertension syndrome by dysfunction of right ventricle through H1 receptor in broilers.

关 键 词：扑尔敏 西咪替丁 组胺 右心功能 肉鸡 

分 类 号：S852.2[农业科学—基础兽医学]