新型生物复合材料——骨髓干细胞结合多孔磷酸三钙的骨再生  

Bone regeneration study by new bioactive composite material——Bone marrow stem cells combining with porous tricalcium phosphate

作　　者：干耀恺[1] 戴尅戎[1] 汤亭亭[1] 张蒲[1] 袁振[1] 李慧武[1] 孙晓江[1] 谢鑫荟[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院骨科,上海200011

出　　处：《复合材料学报》2008年第6期111-117,共7页Acta Materiae Compositae Sinica

基　　金：上海市科委国际合作重点课题(045407008);上海市骨科内植物重点实验室建设基金(08DZ2230330)

摘　　要：将自体富集骨髓间充质干细胞(MSCs),快速复合多孔β磷酸三钙(β-TCP),构建生物活性复合材料(MSCs/β-TCP)。通过分层随机分组对照比较了MSCs/β-TCP与单纯β-TCP及自体骨在羊腰椎后路融合的效果。测定富集后碱性磷酸酶染色为阳性的细胞集落单位(CFUs/ALP+,即MSCs数量)变化情况。用扫描电镜观察了MSCs与β-TCP短期复合情况。通过X线、CT扫描和组织学观察对比植入材料成骨情况及转归。结果表明,富集后MSCs数量增加约3.9倍,短期复合2 h后MSCs即可在多孔β-TCP内壁黏附;X线检查,MSCs/β-TCP组与自体骨组的融合区逐渐有中高密度影形成,CT扫描测定MSCs/β-TCP组与自体骨组的融合率,分别为92%和100%,明显高于单纯-βTCP组(58%)。硬组织形态计量学证实,复合材料MSCs/-βTCP与自体骨的骨再生能力相当,优于单纯β-TCP材料。By combining the enrichment technique of autologous bone marrow mesenchymal stem cells （MSCs） and the rapid hybrid technique with porous β-tricalcium phosphate （β-TCP）, the bioactive composite （MSCs/β-TCP） was constructed. And with randomization and control principles, the enriched MSCs/β-TCP was identified for its osteogenesis function in goat spinal fusion experiment with the control of β-TCP alone and the control of autograft. The change of the number of MSCs post- enrichment was estimated by counting the colony - forming units which express alkaline phosphatase （CFUs/ALP^＋ ）. The early stage of cell-scaffold hybrid was observed by scanning electron microscope （SEM）. By X-ray, CT scan and histologic analysis, the osteogenesis of three sorts of materials and their transformations in vivo were evaluated. The results show that the concentration of MSCs after enrichment technique increases about 3.9 times, and the MSCs adhere to the inside wall of porous β-TCP only with 2 h hybrid. X-ray identifies that the moderate density shadows can be found gradually in the fusion site of group MSCs/β-TCP and group autograft. CT scan shows the fusion rates of group MSCs/β-TCP and autograft were 92% and 100% respectively, which were higher than those of group β-TCP alone （58%）. The undecalcified section confirms that the osteogenesis ability of bioactive composite （MSCs/β-TCP） can compare with autologous bone graft in goat spinal fusion, and is better than that of β-TCP alone.

关 键 词：生物复合材料 间充质干细胞 骨再生 

分 类 号：TB332[一般工业技术—材料科学与工程]