小胡杨小孢子发生及微管骨架变化  

Organization of microtubule during microsporogenesis in Populus simonii × P. euphratica

作　　者：张正海[1,2] 康向阳[1] 刘明虎[3] 段乌拉[3] 

机构地区：[1]北京林业大学林木花卉遗传育种教育部重点实验室 [2]中国农业科学院蔬菜花卉研究所 [3]中国林业科学研究院沙漠林业试验中心

出　　处：《北京林业大学学报》2008年第6期36-40,共5页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(30471407);北京林业大学研究生自选课题基金项目(04jj008)

摘　　要：利用间接免疫荧光结合DAPI(4′,6-diamidino-2-phenylindole)染色方法,进行了小胡杨小孢子母细胞减数分裂过程中微管骨架变化和染色体行为的研究。结果表明:①小胡杨小孢子发生过程中细胞内微管骨架呈动态变化过程,中期Ⅱ形成平行纺锤体和垂直纺锤体;末期Ⅱ未观察到典型的成膜体结构,同时型胞质分裂由子核间微管系统的相对界面发生,胞质分裂后形成四边形和四面体型四分体。②小胡杨小孢子母细胞减数分裂过程中还存在各种异常细胞学现象,中期Ⅰ和中期Ⅱ存在落后染色体;中期Ⅱ相互平行纺锤体发生联合;中期Ⅱ和后期Ⅱ孢母细胞两个纺锤体间的胞质会出现裂沟;四分体时期存在三分体和二分体等,说明由于远缘杂交而造成小胡杨染色体的异质性,并在减数分裂过程中得到体现。In search of a new method to illustrate the microsporogenesis, this microscopy with DAPI （4＇, 6-diamidino-2-phenylindole） staining to pattern and chromosomes behavior during microsporogenesis of Populus study uses indirect immunofluorescence observe the microtubule cytoskeleton simonii x P. euphratica. The results indicated that microtubule cytoskeleton was involved in many key processes of microsporogenesis. Spindles of metaphase Ⅱ were mutually parallel or perpendicular. No typically phragmoplasts were observed at telophase Ⅱ , while cytokinesis occurred simultaneously among the radial microtubules systems which expanded from the sister nuclei and non-sister nuclei. The tetrad was shaped chromosomes were distinguished in anaphase Ⅰ and anaphase Ⅱ observed during microsporogenesis, such as second meiosis, furrow occurred between the dyad were found together with tetrad at tetrad Key words immunofluorescence ; meiosis ; in a tetrahedral or isobilateral pattern. by DAPI staining. Abnormal meiosis were Lag also fused spindles at metaphase Ⅱ , asynchronous division of the mutually parallels at the second division, as well as triad and stage. microtubule ; microspore ; Populus simonii x P. euphratica

关 键 词：免疫荧光 减数分裂 微管 小孢子母细胞 小胡杨 

分 类 号：S792.11[农业科学—林木遗传育种] S718.46[农业科学—林学]