生长激素对人胰腺癌荷瘤裸鼠移植瘤与小肠黏膜IGF—Ⅰ和IGF-Ⅱ的影响  

Impact of growth hormone on the pathways of IGF- Ⅰ and IGF- Ⅱ in pancreatic cancer and in small intestine mucosa of the host

作　　者：石毅[1] 孙跃明[1] 白剑锋[1] 陆文熊[1] 傅赞[1] 赵翰林[1] 苗毅[1] 

机构地区：[1]南京医科大学第一附属医院普外科,210029

出　　处：《中华普通外科杂志》2008年第11期846-849,共4页Chinese Journal of General Surgery

基　　金：江苏省“135”基金重点资助项目（08）

摘　　要：目的探讨生长激素（GH）对人胰腺癌荷瘤裸鼠移植瘤及小肠黏膜组织胰岛素样生长因子Ⅰ、Ⅱ（IGF-Ⅰ、-Ⅱ）的影响。方法用原位杂交法检测胰岛素样生长因子Ⅰ型、Ⅱ型受体（IGFR—Ⅰ、-Ⅱ）mRNA在胰腺癌细胞株SW-1990、移植瘤的表达；裸鼠成瘤后随机分为注射生长激素的实验组（GH组）及注射生理盐水的对照组（NS组），荷瘤裸鼠在依次注射GH后第1、2、6及24h取材，Western方法观察移植瘤及宿主小肠黏膜细胞中IGF—Ⅰ、-Ⅱ的表达变化。结果IGFR—Ⅰ，ⅡmRNA在胰腺癌呈较强表达；与对照组相比，注射GH后1h移植瘤IGF—Ⅰ、IGF-Ⅱ差异无统计学意义（P〉0．05）；而在小肠黏膜，IGF—Ⅰ表达在GH刺激后1、2，6h有明显提高（1h：0．33±0．05，P〈0．05；2h：0．34±0．04，P〈0．05；6h：0．34±0．05，P〈0．05），IGF-Ⅱ在1h有所增强（0．36±0．05，P〈0．05）；IGF—Ⅰ、-Ⅱ的表达在24h时与对照组的差异均无统计学意义。结论作为合成代谢剂的GH能上调荷瘤宿主小肠黏膜局部IGF—Ⅰ、-Ⅱ，但对失去正常调控的肿瘤则作用不明显。Objective To study the impact of exogenous growth hormone （GH） on the levels of insulin-like growth factor- Ⅰ and - Ⅱ （ IGF- Ⅰ , - Ⅱ ） of the pancreatic cancer tissue and the small intestine mucosa of the host. Methods In situ hybridization was performed on pancreatic cancer cell lines （SW-1990） and inoculation tumor of the host to determine the location of the mRNA transcript encoding IGF R- Ⅰ ,-Ⅱ. Athymic nude Balb/c mice were inoculated with SW-1990 cells. After inoculated tumors have become palpable, animals were randomized to receive GH （4 mg/kg once daily for 2 weeks） versus saline control. After the animals were killed at time point, tissues （tumor and small intestine） were rapidly incised for subsequent immune blotting analysis. Results Strong IGF R-Ⅰ ,-Ⅱ mRNA hybridization signal could he detected in pancreatic cancer cell. There was no statistically significant difference between the level of IGF- Ⅰ , Ⅱ in the tumor of the GH and NS groups after 1 hours of GH injection （P 〉 0. 05 ）. GH augmented the expression of IGF-Ⅰ （1 h： 0.33 ±0.05, P〈0.05; 2h： 0.34 ±0.04, P〈0.05; 6 h： 0.34±0.05, P〈0.05）, -Ⅱ （1 h： 0.36±0.05, P〈0.05） in the small intestine mucosa of the host. Conclusions The expression of IGF- Ⅰ , Ⅱ in the small intestine mucosa of the host was elevated by GH, but not in the inoculation tumor in vivo. The discrepancy of GH-IGF pathway between inoculation tumor and small intestine of the host may help to explain the phenomena that GH doesn＇t accelerate growth of pancreatic

关 键 词：生长调节素类 胰腺肿瘤 生长激素 受体 IGF型 

分 类 号：R686[医药卫生—骨科学]