荧光定量PCR检测pN0期结直肠癌淋巴结中CK20mRNA的表达及其临床意义  

CK20mRNA expression detected by FQ-PCR and its clinical significance in lymph nodes from pN0 colorectal cancer patients

作　　者：陈维香 费绍华 祝蕾[2] 伍建 丁君 陶祥 

机构地区：[1]上海市公利医院病理科,200135 [2]复旦大学附属肿瘤医院分子病理室

出　　处：《中华普通外科杂志》2008年第11期863-865,共3页Chinese Journal of General Surgery

基　　金：上海市浦东新区青年基金资助课题（PW2005B-1）

摘　　要：目的探讨N0期大肠癌淋巴结微转移的检测及其临床意义。方法采用荧光定量PCR方法检测62例行根治术的N0期大肠癌患者的548枚淋巴结中细胞角蛋白20（cytokeratin，CK20）mRNA的表达。结果本组62例N0期大肠癌患者的548枚淋巴结中，有24例（39％）共55枚（10．0％）淋巴结中检出微转移。其中第Ⅰ、第Ⅱ、第Ⅲ组淋巴结微转移率分别是15．8％、5．0％和3．3％。微转移与患者年龄、性别、肿瘤大小、肿瘤部位和分化程度等无关，但与肿瘤浸润深度相关（x^2=5．462，P〈0．05）。结论检测N0期大肠癌淋巴结中CK20mRNA的表达可对精确临床分期、了解淋巴结微转移分布及制定合理的治疗方案提供一定的理论依据。Objective To study lymph node micrometastasis in NO colorectal cancer patients and its clinical significance. Methods In this study, 548 lymph nodes obtained from 62 cases of No colorectal cancer undergoing curative operation were examined by fluorescent quantity polymerase chain reaction assay for the expression of cytokeratin 20 （CK20） mRNA. Results Micrometastasis was detected in 55 lymph nodes （ 10. 0% ） of 24 cases （39%）. According to lymph node anatomical locations, micrometastasis was identified in 15.8%, 5.0% and 3.3% lymph nodes in group Ⅰ , Ⅱ and Ⅲ （grouped by the distance from the tumor）, respectively. Micrometastasis was correlated with invasion depth of primary tumor, but was not related to gender, age, tumor size, tumor site and differentiation. Conclusions The expression of CK20mRNA in lymph nodes in patients with NO coloreetal cancer could be used to improve the accuracy of clinical staging, and provide information for rational adjuvant therapy.

关 键 词：结直肠肿瘤 角蛋白 肿瘤转移 淋巴结 微转移 

分 类 号：R686[医药卫生—骨科学]