骨髓间充质干细胞移植对猪心肌梗死后TGF-β1、MMP-1的影响  

The Experimental Study on the Effect of TGF-β1,MMP-1 after Autologous Mesenchymal Stem Cells' Transplantation in Porcine Myocardial Infarction

作　　者：郑琳[1] 沈振亚[2] 陈鑫[1] 李科[3] 李厚怀[3] 藤小梅[2] 惠杰[2] 

机构地区：[1]南京医科大学附属南京第一医院心胸外科,南京210006 [2]苏州大学附属第一医院心血管外科,江苏苏州215006 [3]江苏省肿瘤医院胸外科,南京210000

出　　处：《中国现代医生》2008年第33期44-46,F0003,共4页China Modern Doctor

摘　　要：目的研究经冠状动脉注射自体骨髓间充质干细胞对心肌梗死后左心室舒缩功能和血清TGF-β1、MMP-1的影响。方法10只太湖梅山猪通过介入栓塞冠状动脉建立心肌梗死动物模型,随机分为移植组,梗死4周后注射MSCs;对照组,梗死4周后注射DMEM;每组5只。抽取实验猪髂骨骨髓体外分离、培养、纯化,得到MSCs,通过介入法将约1.0×107的MSCs注入梗死4周的实验猪冠状动脉栓塞远端。观察移植后1周、4周时的左心室舒缩功能变化,观察不同时点血清TGF-β1和MMP-1的含量。结果MSCs移植后4周,+dp/dtmax、-dp/dtmax较移植前和对照组明显提高(P<0.01);干细胞移植后不同时点的血清TGF-β1含量不断减少但到2周时达谷值(P<0.01),对照组仅有轻度的下降趋势;干细胞移植后1周时的血清MMP-1含量增加,2周后达到高峰(P<0.01),而对照组则稳定维持在移植前水平。结论经冠脉自体MSCs移植能够明显改善心肌梗死后左心室的舒缩功能,降低TGF-β1在血清中的水平,同时促进MMP-1的合成、分泌。Objective To evaluate the left ventricle function of myocardial infracted porcine which cured by autologous bone messenchymal stem cells（MSCs） transplantation by injection of coronary artery. Methods The 10 myocardial infarcted porcines which produced by percutaneous coronary embolism were divided into 2 groups：transplantation group, which cured by MSCs;control group, which cured by DMEM;every group had 5 poreines. The bone marrow were drawed from iliae bone of porcine and separated, cultured and purified in vitro, then we could oblain MSCs, and injected it into the coronary artery of myocardial infarcted porcines by cardiac cathete. To observe the change of left ventricular diastole and contraction function 1 week and 4 weeks post-transplantation,and to detect the content of serum MMP-1 and TGF-β1 at fixed time. Results At 4 weeks post-transplantation,transplantation group had significant improvement in ＋dp/dtmax and -dp/dtmax （P〈 0.01 ）; Compared with control group,the content of serum MMP-1 increased significantly at 1 week after MSCs＇ transplantation,reaching the maximum 2 weeks later（P〈 0.01 ）. At the same time,the content of serum TGF-β1 had been decreasing continually until 2 weeks post- trans- plantation（P〈 0.05 ）. Conclusion The therapy of autologous hone messenchymal stem cells＇ transplantation can improve evidently the function of left ventricular diastole and contraction. Stem cells＇ transplantation can reduce the level of TGF-β1 in serum, meanwhile accelerate synthesiss and secretion of serum MMP-1.

关 键 词：心肌梗死 骨髓间充质干细胞 

分 类 号：R542.2[医药卫生—心血管疾病]