Thy1．1干细胞移植能抑制动脉损伤后内膜增生  

The Influence of Thy-1.1 Stem Cell Transplantation on Neointimal Formation of Injured Artery

作　　者：董少红 刘华东 姜昕 

机构地区：[1]暨南大学第二临床医学院,深圳市人民医院心内科,广东深圳518020

出　　处：《中华高血压杂志》2008年第11期1003-1008,共6页Chinese Journal of Hypertension

基　　金：广东省卫生厅基金资助项目(编号:A2005617)

摘　　要：目的探讨大鼠Thy-1．1干细胞局部移植对大鼠颈总动脉球囊损伤后内膜增生的影响，评价干细胞移植对再狭窄的作用。方法雌性SD大鼠60只随机分为2组，每组30只，即于细胞移植组：于颈总动脉球囊损伤后即刻将约5×10^6 Thy-1．1干细胞注入至损伤血管局部；对照组：颈总动脉球囊损伤后局部注入等量生理盐水。另有30只4～6周龄雄性SD大鼠提供骨髓Thy-1．1干细胞。2组各于术后即刻、3d、7d、14d、28d取损伤血管段，病理组织学方法观察细胞增殖情况，原位杂交方法观察移植细胞的定植、分化情况，RT-PCR方法分析内皮型一氧化氮合酶（eNOS）的表达情况。结果干细胞移植组内膜面积低于对照组[I／M，干细胞移植组：2．06±0．28比对照组2．42±0．19，P〈0．05]，干细胞移植组内皮型一氧化氮合酶（eNOS）的表达明显高于对照组[-eNOSmRNA，干细胞移植组：0．44±0．06比对照组0．35±0．02，P〈0．0S]，干细胞移植组原位杂交图片中可见内皮细胞有移植的异体细胞。结论Thy-1．1干细胞局部移植可促进大鼠颈总动脉球囊损伤后再内皮化，抑制内膜增生，预防血管成形术术后再狭窄。Objective To study the effect of Thy-1.1 stem cell transplantation on endothelial hyperplasia and restenosis. Methods Thirty 4--6 weeks male SD rats were sacrificed to obtain the Thy-1.1 stem cells. Carotid artery were injured by ballon in sixty female SD rat＇s were randomized to receive stern cell transplantation （ 5 × 10^6 Thy-1.1, n= 30） or saline approach（n= 30 ）. About 5 X 106 Thy-1.1 stem cells were injected into the injured artery after carotid artery injury; while the control rats underwent carotid artery injury and was injected the same amount of saline. The animals were sacrificed, 3, 7, 14, 21 and 28 days after balloon denudation. The samples of carotid artery were harvested for pathological examination, RT-PCR and in situ hybridzation（ISH） were used to detect the transplanted cells in the injured artery. Results The intimal thickness was thinner in stem cell transplantation group （I/M, Stem cell transplantation group： 2.06±0.28 vs control group 2.42±0. 19, P〈0. 05], and eNOS mRNA expression was higher [＇eNOS mRNA, Stem cell transplantation group： 0.44±0.06 vs control group 0.35±0.02, P〈0.05]. The transplanted cells can be tracked by ISH in proliferated endothelial cells. Conclusion Stem cell transplantation accelerates re-endothelialization and decreases neointimal formation after vascular injury, suggesting that Thy-1.1 stem cell transplantation might be a feasible treatment to prevent restenosis after PCI.

关 键 词：骨髓干细胞 Thy-1.1抗原 再狭窄 血管损伤 大鼠 

分 类 号：R543[医药卫生—心血管疾病]