应用PCR方法检测烟粉虱传双生病毒  

Detection of whitefly-transmitted geminiviruses by reverse transcription-polymerase chain reaction

作　　者：叶青静[1] 杨悦俭[1] 王荣青[1] 李志邈[1] 阮美颖[1] 周国治[1] 姚祝平[1] 

机构地区：[1]浙江省农业科学院蔬菜研究所,杭州310021

出　　处：《植物保护》2008年第6期25-28,共4页Plant Protection

基　　金：浙江省科技厅资助项目(2007C32011)

摘　　要：番茄黄化曲叶病毒属于双生病毒,根据其外壳蛋白基因保守序列设计简并引物,对杭州和海宁地区番茄上疑似番茄黄化曲叶病毒进行PCR检测,获得产物克隆到pMD18-T载体中,经测序和序列分析,表明杭州和海宁地区发生的病毒是番茄黄化曲叶病毒,并且两地区病毒核苷酸序列同源性达97.5%。Tomato yellow leaf curl virus （TYLCV） is a geminivirus transmitted by the whitefly Bemisia tabaci. A pair of degenerate primers was designed according to the conserved sequence of coat protein genes of TYLCV. The tomato samples showing TYLCD-like symptoms were collected from Hangzhou and Haining and detected by PCR for virus presence. PCR products （about 361bp） were then cloned into the vector pMD18-T. Sequence analysis indicated that the nucleotide sequences of the amplified products from Hangzhou and Haining have 97.5% identity, and they share the highest sequence identity with TYLCV, indicating these samples were infeeted by TYLCV.

关 键 词：番茄黄化曲叶病毒 PCR 检测 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]