黑龙江省水稻纹枯病菌的致病力分化与AFLP分析  

Pathogenic differentiation and AFLP analysis of the rice sheath blight strains from Heilongjiang

作　　者：范文艳[1,2] 文景芝[2] 金丽娜[1] 马建[1] 陈瑾[1] 

机构地区：[1]黑龙江八一农垦大学植物科技学院,大庆163319 [2]东北农业大学植物保护学院,哈尔滨150030

出　　处：《植物保护》2008年第6期57-61,共5页Plant Protection

基　　金：黑龙江省高等学校青年学术骨干支持计划项目(1152G022)

摘　　要：为了明确黑龙江省水稻纹枯病菌遗传多样性,为水稻抗病育种和水稻纹枯病的综合防治提供依据。本文对采自13个水稻种植地区的29个水稻纹枯病菌菌株进行了致病力测定和AFLP分析。结果表明9对AFLP引物对供试菌株扩增出396条带,其中多态性带187条,占总扩增带数的47.22%。黑龙江省水稻纹枯病菌的遗传距离变化在0.50～0.92之间,平均为0.71,群体遗传多样性较为丰富。UPGMA法可以将供试菌株分成4个AFLP聚类组群(Ⅰ、Ⅱ、Ⅲ和Ⅳ),相同地理来源的菌株基本上聚集在同一组群内,表明AFLP类群划分与菌株的地理来源有较强的相关性。黑龙江省水稻纹枯病菌致病性分化较为明显,并且AFLP类群划分与菌株的致病性鉴定之间存在一定相关性。To understand the pathogenicity and genetic diversity of Rhizoctonia solani in Heilongjiang, and provide the basis for disease-resistance breeding and integrated control of the rice sheath blight, 29 isolates of the pathogen from 13 rice-growing areas of Heilongjiang were tested for identification of their pathogenicity and analysis of genetic diversity by using AFLP technology. Using 9 pairs of AFLP primers, a total of 396 bands were amplified, of which, 187 （47.22%） were polymorphic. The genetic distances of 29 isolates based on AFLP markers ranged between 0.50 --0.92, averaged at 0.71. The results showed that the genetic diversity among the isolates tested was high. The tested isolates could be classified into 4 AFLP groups （ Ⅰ , Ⅱ , Ⅲ and Ⅳ）, and most of the isolates from the same area clustered in the same group by using the UPGMA method. The analyses showed that there were obvious correlations between AFLP clustering groups and geographical origins of the isolates. The results showed that there existed obvious differentiation of the pathogenicity of R. solani in Heilongjiang Province, and the virulence variation was generally related with AFLP groups.

关 键 词：水稻纹枯病菌 AFLP 致病力 遗传多样性 

分 类 号：S435.111.42[农业科学—农业昆虫与害虫防治]