隔物温和灸联合甲氨蝶呤对RA模型大鼠关节肿胀及IL-1β、TNF-α的影响  

Influence of Indirect Moxibustion plus Methotrexate on Joint Swelling,IL-1β and TNF-ɑ in a Rat RA Model

作　　者：李建武[1] 刘建民[2] 

机构地区：[1]武汉市中医医院针灸科,湖北430014 [2]湖北中医学院,2006级博士生武汉430065

出　　处：《上海针灸杂志》2008年第11期32-34,共3页Shanghai Journal of Acupuncture and Moxibustion

基　　金：武汉市卫生局课题(武卫[2004]241号)

摘　　要：目的观察隔物温和灸联合甲氨蝶呤对RA模型大鼠关节肿胀及IL-1β、TNF-α的影响。方法成功制作RA大鼠模型40只,随机分为模型组、甲氨蝶呤治疗组(MTX组)、隔物温和灸治疗组(温灸组)、隔物温和灸+甲氨蝶呤治疗组(温灸+MTX组)4组,并设正常同龄大鼠对照;各组经过相应处理后,采用ELISA酶联免疫法测定大鼠血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)含量,观测并对比MTX组、温灸组、温灸+MTX组对RA模型大鼠IL-1β、TNF-α含量及跖围变化的影响。结果与正常组比较,模型组IL-1β、TNF-α含量及跖围变化差异有统计学意义(P<0.05或P<0.01);与模型组相比,温灸组、温灸+MTX组的IL-1β、TNF-α含量及跖围变化均显示出组间差异性(P<0.05或P<0.01);温灸组、温灸+MTX组两组间IL-1β、TNF-α含量及跖围变化无组间差异(P>0.05)。结论隔物温和灸能抑制RA大鼠血清IL-1β、TNF-α分泌水平,减轻RA大鼠足跖关节肿胀程度;与MTX联合应用能够使作用加强,为临床RA治疗方案的选择提供了科学依据。Objective To investigate the influence of indirect moxibustion plus methotrexate on joint swelling, IL-1 β and TNF-α in a rat RA model. Methods A rat RA model was successfully made. They were randomly allocated to 4 groups： model, methotrexate （MTX）, indirect moxibustion and indirect moxibustion plus MTX. Normal rats of the same age were used as a control. After each group was treated accordingly, serum interleukin-6 （IL-6） and tumor necrosis factor-ix （TNF-α） contents were measured by enzyme linked immunosorbent assay （ELISA）. The influences on IL-6 and TNF-α contents and metatarsus circumference were compared between the MTX, indirect moxibustion and indirect moxibustion plus MTX groups. Results There were statistically significant differences in IL-1β and TNF-α contents and metatarsus circumference between the normal and model groups （ P 〈 0.05 or P 〈 0.01 ）. There were also statistically significant differences in IL-1 β and TNF-α contents and metatarsus circumference between the model and indirect moxibustion or indirect moxibustion plus MTX groups （ P 〈 0.05 or P 〈 0.01 ）. There were no statistically significant differences in IL-1β and TNF-α contents and metatarsus circumference between the indirect moxibustion and indirect moxibustion plus MTX groups （ P 〉 0.05 ）. Conclusion Indirect moxibustion can decrease serum IL-1β and TNF-α levels and reduce metatarsal joint swelling in RA rats. The combined use of it and MTX can improve the effect and provides a scientific basis for clinical selection of a RA protocol.

关 键 词：关节炎 类风湿 针灸疗法 间接灸 IL-1Β TNFα- 大鼠 

分 类 号：R2-03[医药卫生—中医学]