超低温冷冻对日本鳗鲡精子酶活性的影响  

Cryopreservational effects on the spermatic enzyme's activities of Japanese ell(Anguilla japonica)

作　　者：黄晓荣[1,2] 章龙珍[1,2] 庄平[1,2,3] 江琪[1,2] 刘鉴毅[1] 姚志峰[1,2] 张涛[1] 冯广朋[1,2] 赵峰[1,2] 

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]上海海洋大学生命科学与技术学院,上海200090 [3]上海市高校水产养殖学E-研究院,上海200090

出　　处：《海洋渔业》2008年第4期297-302,共6页Marine Fisheries

基　　金：国家科技基础条件平台(2006DKA30470-004);中央级公益性科研院所基本科研业务费专项资金项目(2007T02);上海市高校水产养殖学E-研究院建设项目(E03009)

摘　　要：研究超低温冷冻保存(-196℃)对日本鳗鲡精子内总ATP酶、肌酸激酶(CK)、琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)等酶活性的影响。运用试剂盒分别测定了冷冻前后日本鳗鲡精子内酶活性的变化。结果表明,经过超低温冷冻保存后,日本鳗鲡精子的活力下降,精子内GR活性显著升高(P<0.05),酶活性从冻前的358.52±45.65 U/L上升到646.30±70.30 U/L;其它几种酶的活性均显著下降(P<0.05),总ATP酶、CK和SDH的活性分别从冻前的3.14±0.61 U/ml、17.10±3.51 U/ml和32±5.94 U/ml下降到1.83±0.43 U/ml、7.33±1.74 U/ml和21±1.41 U/ml,LDH、SOD和CAT活性分别从冻前的2 266.67±313.25 U/L、220.47±32.94 U/ml和48.51±5.94U/ml下降到1 195.91±198.51 U/L、84.16±22.11 U/ml和21.8±4.14 U/ml。超低温冷冻对日本鳗鲡精子酶活性和精子活力均有较大影响。Present paper dealed with the study concerning the effects of cryopreservation（ -196℃ ） on the enzyme＇s activities （including total ATP, SDH, LDH, SOD, CAT and GR） in spermatozoa of Japanese eel （Anguilla japonica） . By means of reagent box, the changes of enzyme＇ s activities of spermatozoa mentioned above have been respectively analysed. The results showed： the activities of spermatozoa dropped after cryopreservation, GR activities increased significantly（ P 〈0.05 ）, the activities added from 358.52 ± 45.65 U/L to 646.30 ± 70.30 U/L, other enzyme＇s activities descended significantly（P 〈 0.05）. The activities of total ATP, CK, SDH dropped from 3.14 ±0.61 U/ml, 17.10 ±3.51 U/ml and 32 ±5.94 U/ml to 1.83 ±0. 43 U/ml, 7.33 ± 1.74 U/ml and 21 ± 1.41 U/ml respectively, the activities of LDH, SOD and CAT declined from 2266.67 ±313.25 U/L, 42.65 ±1.56 U/ml and 48.51 ±5.94 U/ml to 1195.91±198.51 U/L, 84.16 ±22.11 U/ml and 21.8±4. 14 U/ml respectively. Cryopreservation had a great effect on the enzyme＇s activities and spermatozoa＇ s energy of AnguiUa japonica.

关 键 词：超低温冷冻 日本鳗鲡 精子 酶活性 

分 类 号：S917[农业科学—水产科学]