五爪金龙总黄酮的超声提取方法及其鉴定  

Ultrasonic Extraction and Identification of Flavonoids of Ipomoea cairica

作　　者：赵则海[1] 韦昌挺[1] 陈庆华[1] 王治辉[1] 

机构地区：[1]肇庆学院生命科学学院,广东肇庆526061

出　　处：《林业科技开发》2008年第6期88-90,共3页China Forestry Science and Technology

基　　金：肇庆市科技创新计划项目(编号:2008G23);肇庆学院自然科学基金项目(编号:0544)

摘　　要：采用正交试验法研究了五爪金龙叶中总黄酮的超声提取最佳工艺,并测定其含量。五爪金龙样品用石油醚脱去脂类及部分色素,总黄酮得率最高的提取方法为:乙醇体积分数为80%,超声提取60 min,乙醇溶液体积(mL)与干粉质量(g)比例为40∶1。按最佳超声提取条件,五爪金龙的粗黄酮得率为8.75%,总黄酮提取率为1.216%。通过颜色反应和紫外可见光谱分析,初步确定五爪金龙叶中的黄酮类化合物主要有黄酮、黄酮醇、二氢黄酮和二氢黄酮醇类化合物。The ultrasonic extraction technique of flavonoids from L cairica leaves was studied by orthogonal test. Meanwhile, contents of flavonoids were measured. After leaf powder of L cairica had been shucked lipid and pigment by ligroine, the optimum extraction condition was determined as follows： 80% ethanol as extracting solvent, ultrasonic extraction time is 60 min, the volume ratio of solvent to the mass of rawmaterial is 40： 1. With the optimum ultrasonic extraction condition, the yield of flavonoids in leaves of L cairica reached 8.75 %. The extraction yield of flavonoids was 1. 216 %. L cairica flavonoids was identified preliminarily as flavone, flavonol, flavanone and flavanonol by color reaction and ultravio-let-visible spectrum.

关 键 词：五爪金龙 超声提取 黄酮类化合物 定性鉴定 藤本植物 

分 类 号：S451.2[农业科学—植物保护] TS225.19[轻工技术与工程—粮食、油脂及植物蛋白工程]