大孔吸附树脂纯化小丛红景天总黄酮的初步研究  

Preliminary Study on Purification Technics of Total Flavonoids in Rhodiola dumulosa by Macroporous Resin

作　　者：谭林[1] 郭增军[1] 徐颖[1] 

机构地区：[1]西安交通大学医学院药学系,陕西西安710061

出　　处：《中药材》2008年第11期1740-1743,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:筛选纯化小丛红景天总黄酮的最佳树脂,并对影响纯化的各种主要因素进行初步研究,使纯化工艺达到最优化。方法:采用静态吸附/解吸与动态吸附/解吸相结合的方法,用紫外-可见分光光度法测定小丛红景天总黄酮的含量来对工艺进行评价。结果:D101大孔吸附树脂纯化效果最好,其最佳工艺为:上样药液总黄酮浓度为3.625 mg/ml,相当于原生药0.241 g/ml,吸附速率为2BV/h,解吸液乙醇浓度为95%,解吸速率为2BV/h,洗脱剂用量为5倍柱体积,纯度可以达到48.5%。结论:D101大孔吸附树脂吸附性能最好,适于小丛红景天总黄酮的初步纯化。Objective：To choose the best macroporous resin for purifying total flavonoids in Rhodiola dumulosa and study various factors affecting purification of resin in order to optimize the technics. Methods：Using methods combining static adsorption with desorption and dynamic adsorption with desorption, evaluating the technics by determination of total flavonoids in Rhodiola dumulosa using ultraviolet and visible spectrophotometry. Results ：The best maeroporous resin was D101. The optimized technics were as follows：sample total flavonoids concentration was 3. 625 mg/ml, adsorption velocity was 2 BV/h, desorption ethanol concentration was 95%, desorption velocity was 2 BV/h, eluant volume was 5 BV, the purity of total flavonoids could reach 48.5%. Conclusions ： Purification performance of D101 macroporous resin is the best, and it is fit for the preliminary, purification of total flavonoids in Rhodiola dumulosa.

关 键 词：小丛红景天 大孔吸附树脂 总黄酮 工艺优化 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]