子宫颈癌患者血清中半乳凝素9的检测及临床意义  

Serum galectin-9 in cervical cancer

作　　者：梁美艳[1] 鲁艳明[1] 张瑶[1] 张淑兰[1] 

机构地区：[1]中国医科大学盛京医院妇产科,沈阳110004

出　　处：《中华医学杂志》2008年第39期2783-2785,共3页National Medical Journal of China

基　　金：国家自然科技基金资助项目（30672220）

摘　　要：目的检测子宫颈癌患者血清中半乳凝素9（Gal-9）分析其临床意义。方法收集29例子宫颈浸润癌（平均年龄45岁），45例上皮内瘤样病变（CIN）和26例正常妇女的血清（平均年龄均为40岁），应用ELISA方法检测Gal-9的表达，浸润癌组同时检测C反应因子（CRP）和血管内皮生长因子（VEGF）比较相关性。根据血清检测Gal-9结果，选择其中4例子宫颈癌组织，应用免疫组化法比较Gal-9蛋白的差异。结果子宫颈癌组患者血清中Gal-9的表达为27．15ng／L，正常组为17．43ng／L，CIN组为21．20ng／L，浸润癌组血清中Gal-9的表达与CRP和VEGF呈正相关。血清高表达Gal-9的肿瘤组织中Gal-9蛋白的表达高于低表达者。结论血清中Gal-9的产生与子宫颈癌细胞有关。Gal-9在子宫颈癌的形成和侵袭过程中发挥保护作用。Objective To investigate the expression level of Galectin-9 （Gal-9） in the serum of cervical cancer patients and analyze the clinic significance of it. Methods Collected sera from 29 patients with cervical cancer, 45 patients with cervical inter-epithelial lesion （CIN） and 26 normal females. Determination of Gal-9 was performed by ELISA, and in the group of patients with cervical cancer, these results were compared with the serum vascular endothelial growth factor （VEGF）and C-reactive protein （CRP）. Then selecting four patients from the invasive cervical cancer group performed Gal-9 retrospective immuno-staining analysis. Results Gal-9 concentration was 27. 15 ng/L in the group of patients with cervical cancer that was significantly higher compared with the patients with CIN （21.20 ng/L） and the normal females（ 17.43 ng/L）, and the Gal-9 concentration was significantly correlated with both VEGF and CRP in cervical cancer group. Two of these patients, who had a higher Gal-9 serum level, shown more intense staining and the other two patients, with lower serum level of Gal-9, had moderate immunostaining, suggesting that at least part of serum Gal-9 might be produced by cervical cancer tissue. Conclusions Gal- 9 might play a role in the progression and invasion of cervical cancer and warrants further study.

关 键 词：子宫颈肿瘤 半乳凝素9 C反应因子 血管内皮生长因子 

分 类 号：R686[医药卫生—骨科学]