局灶性缺血性脑卒中大鼠海马Notch信号通路的动态表达  

Dynamic changes of the expression of Notch and Jagged in the hippocampus after focal ischemia： experiment with rats

作　　者：郭怡菁[1] 张志珺[1] 王少华[1] 隋毓秀[1] 孙奕[1] 滕皋军[2] 

机构地区：[1]东南大学附属中大医院神经内科 东南大学脑血管病研究所,南京210009 [2]东南大学分子影像研究中心

出　　处：《中华医学杂志》2008年第39期2803-2805,共3页National Medical Journal of China

基　　金：国家自然科学基金资助项目（30570656、30600206）

摘　　要：目的观察局灶性缺血性脑卒中大鼠海马Notch信号通路的表达变化，初步探讨该通路在局灶性缺血性脑卒中后神经再生的调控作用。方法大脑中动脉阻塞（MCAO）法建立大鼠局灶性脑缺血模型，通过Western印迹法和实时RT—PCR法分别于卒中后19d和28d检测各实验组及对照组大鼠海马Notch1及其配体Jagged1的蛋白、基因表达。结果卒中后19d，缺血组Notch1蛋白和基因表达均分别高于对照组（均P〈0．05），缺血组28d的Notch1蛋白和基因表达显著低于19d的蛋白和基因表达（均P〈0．01）。缺血组19d和28d的Jagged1蛋白表达（P〈0．05）和基因表达（P〈0．05）高于对照组，但28d的Jagged1蛋白和基因表达与19d相比差异无统计学意义（均P〉0．05）。结论局灶性缺血性卒中大鼠Notch信号通路呈现动态表达变化，提示该通路可能参与了对卒中后内源性神经前体细胞增殖、分化的调控。Objective To detect the expression patterns of Notch1 and Jagged1 in the hippocampus of focal ischemia and find out whether the Notch pathway is involved in the regulation of the differentiation of the proliferated neural precursor ceils in the dentate gyrus under ischemic conditions. Methods 42 SD rats were randomly divided into 3 equal groups ： middle cerebral artery occlusion （ MCAO ） group undergoing MCAO by intraluminal thread technique so as to cause focal cerebral infarction, sham operation group, and normal control group. 19 and 28 days after the operation 6 rats in each group were killed with their hippocampuses taken out. The protein and mRNA levels of Notch1 and Jagged1 in hippocampus were assayed respectively by Western blotting and real-time reverse-transcription PCR. Results The protein and mRNA expression levels of Notch1 on the 19th day of the MCAO group were up-regulated, both significantly higher than those of the sham operation and control groups （ all P 〈 0. 05 ）. The protein and mRNA expression levels of Notch1 on the 28th day of the MCAO group were both significantly lower than those of the same group on the 19th day （ both P 〈 0. 01 ）, and were lower than those of the sham operation and control groups on the 28th day, however, not significantly （ all P 〉 0. 05 ）. The protein and mRNA expression levels of Jagged1 on the 19th and 28th days of the MCAO group were significantly higher than those of the sham and control groups （ all P 〈 0. 05 ）, however, there were not significant differences in the protein and mRNA expression levels of Jagged1 on the 19th and 28th days of the MCAO group （all P 〉 0. 05 ）. Conclusion The protein and gene expression of Notch1 and Jagged1 vary dynamically in the hippocampus of focal ischemia, which suggests that the Notch pathway may be a potential mechanism in the regulation of the neurogenesis in the dentate gyrns of hippocampus.

关 键 词：脑梗死 信号传导 神经再生 

分 类 号：R686[医药卫生—骨科学]