化疗所致卵巢早衰动物模型的建立  

Establishment of rat model of chemotherapy-induced premature ovarian failure

作　　者：付霞霏[1] 何援利[1] 

机构地区：[1]南方医科大学珠江医院妇产科,广州510280

出　　处：《广东医学》2008年第12期1952-1954,共3页Guangdong Medical Journal

基　　金：广东省科技计划项目(编号:2007B030501005)

摘　　要：目的探索建立化疗所致卵巢早衰动物模型的方法。方法以Wistar雌性大鼠为研究对象,以5种不同剂量和方案腹腔注射环磷酰胺,观察大鼠一般情况、动情周期、性激素(雌二醇和卵泡刺激素)水平、卵巢重量及形态学、各级卵泡数的变化。结果环磷酰胺可引起大鼠体重明显下降,动情周期紊乱,低雌激素血症,高促性腺激素血症,卵巢重量明显减轻,卵巢间质增生明显,窦前及窦状卵泡数减少,与对照组相比均有明显差异。结论采用腹腔注射环磷酰胺负荷剂量50mg/kg后以8mg/(kg.d)的维持剂量连续注射14d的方案建模,大鼠卵巢功能明显衰退,卵巢组织学及内分泌改变与临床卵巢早衰患者相似。该方法简便易行、成模时间短、成功率高、死亡率低,是建立化疗所致卵巢早衰动物模型的一种较好的方法。Objective To investigate an applicable method of establishment of animal model of chemotherapy - induce premature ovarian failure. Methods Female Wistar rats were injected i. p. with cyclophosphamide（CTX） with different doses and protocals. Body weight, estrous cyclieity, concentration of gonadal hormones（ estrogen and follicle - stimulating hormone） , ovarian weight and histopathology, and number of follicles were examined, and compared with those of control group. Results Cyclophosphamide induced decreased body and ovarian weight, abnormal estrous cyclicity, hypoestrogenemia, hypergonadotrophinemia, decreased preantral and antral follicles. Conclusion Load dose of CTX （50 mg/kg） followed by i.p. 8 mg/（kg · d） for 14 days can damage the ovarian function. The changes in sex hormone and histopathology were similar to those in premature ovarian failure patients. The method can be used to establish the animal model of chemotherapy- induced ovarian failure.

关 键 词：卵巢早衰 化学治疗 动物模型 大鼠 

分 类 号：R711.75[医药卫生—妇产科学] R730.53[医药卫生—临床医学]