Tumstatin肽对视网膜微血管内皮细胞增殖及ERK蛋白表达的影响  

The effect of tumstatin peptide on the proliferation and expression of extra-cellular signal-regulated protein kinase in retinal microvascular endothelial cells

作　　者：黄敏丽[1] 罗国容[2] 陈维平[2] 何少健[2] 陈芳[2] 

机构地区：[1]广西医科大学第一附属医院眼科,南宁530021 [2]广西医科大学组织胚胎学教研室,南宁530021

出　　处：《广东医学》2008年第12期1955-1958,共4页Guangdong Medical Journal

基　　金：广西自然科学基金资助项目(编号:桂科自0640090);广西教育厅博士研究生科研创新基金资助项目(编号:2006105981001D13)

摘　　要：目的观察Tumstatin肽对体外培养的视网膜微血管内皮细胞增殖及细胞外信号调节激酶(ERK)蛋白表达的影响。方法采用MTT比色法测定Tumstatin肽(T8肽)对血管内皮生长因子(VEGF)诱导下的恒河猴视网膜血管内皮细胞(RF/6A细胞)增殖的干预作用;采用Western-blot检测T8肽对VEGF刺激后15,30,45,60min的RF/6A细胞ERK1/2蛋白表达水平的变化。结果T8肽浓度在5μg/ml以上时对RF/6A细胞有抑制作用,且抑制作用随着浓度的增大而增高,各组A值和抑制率比较差异均有显著性(均P<0.05);T8肽可抑制VEGF对RF/6A细胞的促增殖作用;在1～40μg/ml相同浓度条件下,T8肽对VEGF条件下的RF/6A细胞增殖抑制率显著大于无VEGF条件下的抑制率(均P<0.01)。VEGF可刺激RF/6A细胞ERK1/2蛋白表达增加,T8肽可抑制VEGF诱导的RF/6A细胞ERK1/2蛋白的表达。结论Tumstatin抑制视网膜微血管内皮细胞的增殖,机制可能与VEGF信号转导通路上的ERK1/2细胞信号转导通路有关。Objective To study the effects of tumstatin peptide on the proliferation and expression of extra - cellular signal - regulated protein kiuase （ERK） in retinal microvascular endothelial cells. Methods MTI＇ assay was used to examine the proliferation induced by vascular endothelial growth factor（VEGF） in RF/6A cells （ Cell line of retinal microvascular endothelial cell of Macaca rhesus）. The expression of ERK1/2 15, 30, 45 and 60 minutes ＇after tumstatin peptide （T8 peptide） and VEGF treatment was detected by Western blotting. Results Different concentrations of tumstatin peptide （T8 peptide） including 5 ug/ml, 10 ug/ml, 20 ug/ml and 40 ug/ml inhibited the growth of RF/6A, and cellular growth decreased gradually with the increase in tumstatin concentration（P 〈 0.05 ）. T8 peptide inhibited proliferation of RF/6A cells induced by VEGF. Its inhibitory, effect increased with the elevation of T8 peptide concentration（P 〈 0.01 ）. Protein expression of ERK1/2 increased rapidly by VEGF stimulation. Tumstatin inhibited the expression of ERK1/2 induced by VECF in RF/6A cells. Conclusion Tumstatin can inhibit proliferation of retinal microvascular endothelial cells, which is associated with ERK1/2 signaling.

关 键 词：TUMSTATIN 视网膜微血管内皮细胞 增殖 有丝分裂激活蛋白激酶类 

分 类 号：R979.1[医药卫生—药品] R774[医药卫生—药学]