补阳还五汤含药脑脊液对受损皮质神经细胞应激反应的调节作用  

Protective Effect of Buyanghuanwu Decoction in Cerebrospinal Fluid on Damaged Cortical Neurocyte

作　　者：邹纯朴[1] 陈晓[1] 杨柳[1] 

机构地区：[1]上海中医药大学基础医学院内经教研室,上海201203

出　　处：《四川中医》2008年第11期9-12,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：上海市教委课题(05cz05)

摘　　要：目的:观察补阳还五汤含药脑脊液对受损皮质神经细胞应激反应的调节作用。方法:在离体培养5天的大鼠皮质神经细胞中分别加入不同的含药脑脊液,37℃孵育24h,然后加入10μmol/L谷氨酸继续孵育60min。通过MTT法检测细胞生存活力;RT-PCR法检测即早基因的表达;扫描电镜观察细胞形态变化。结果:补阳还五汤含药脑脊液能很好的提高细胞生存活力,抑制即早基因的表达。结论:中药复方补阳还五汤能对抗皮质神经细胞的谷氨酸损伤,其机制可能通过抑制即早基因c-fos、c-jun的表达,防止皮质神经细胞凋亡。Objective： To investigate the protective effect of Buyanghuanwu decoction on damaged cortical neurocyte. Methods： Cerebrospinal fluid with different medicinal content was added into rat＇s cortical neurocytes, which were then incubated at 37℃ for 24 hours, added 10μmol/L aminoglutaminic acid and incubated for 60 mins. The live activity of neurocytes was detected through MTT, and the expression of immediate early genes were detected through RT-PCR, and cell morphological changes were observed through scanning electron microscope. Results： Buyanghuanwu decoction increased the live activity, inhibited the expression of immediate early gene. Conclusion： Buyanghuanwu decoction has protective effects on cortical neurocyte against injury induced by aminoglutaminic acid, through inhibiting the expression of immediate early gene c-fos, c-jun and preventing the apoptosis.

关 键 词：补阳还五汤 脑脊液药理学 缺血性脑血管病 即早基因 

分 类 号：R285.5[医药卫生—中药学]