麝香乌龙丸对兔膝骨关节炎软骨组织病理形态学及MMP-1、TIMP-1表达的影响  

The Effect of Musk-Aconite-Earthworm Pill on Pathomorphology and Expression of MMP-1,TIMP-1 of Cartilage in Rabits with KOA

作　　者：王志文[1] 尹学永[2] 汪福东[2] 张爱国[1] 袁强[1] 谷宁飞[2] 赵鑫[2] 

机构地区：[1]华北煤炭医学院附属医院,河北唐山063000 [2]华北煤炭医学院,河北唐山063000

出　　处：《四川中医》2008年第11期12-14,共3页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:观察麝香乌龙丸对兔膝骨关节炎软骨组织病理形态学、基质金属蛋白酶1(MMP-1)及其抑制剂(TIMP-1)的影响,探讨麝香乌龙丸对关节软骨的保护作用是否与调节MMP-1及TIMP-1的表达有关。方法:30只日本大耳白兔随机分为3组:A正常对照组,B模型对照组,C麝香乌龙丸组。用1.6%木瓜蛋白酶膝关节腔内注射,建立兔膝骨关节炎模型,C组灌胃给药4周后,观察软骨组织形态学的变化及软骨中MMP-1、TIMP-1的表达。结果:C组软骨病理损害程度轻于B组(P<0.05),MMP-1表达明显低于B组(P<0.01),TIMP-1表达高于B组(P<0.01)。结论:麝香乌龙丸能减轻膝骨关节炎关节软骨退行性改变,其对关节软骨的保护作用可能与调节MP-1及TIMP-1的表达有关。Objective： To observe the effect of Musk-Aconite-Earthworm pill on pathomorphology and expression of MMP-1 and TIMP-1 of cartilage in rabbits with KOA, and to explore whether there is certain relation between cartilage protection and expression of MMP-1 and TIMP-1. Methods： A total of 30 healthy male rabbits were randomly divided into three groups： normal control group （group A）, model group （group B）, and Musk-Aconite-Earthworm pill group （group C） . KOA model was established by injecting papain to the right knees of rabbits. Rabbits in group C were intragastrically administered Musk-Aconlte-Earthworm pills for 4 weeks. Then, path-omorphological changes of cartilage and expression of MMP-1 and TIMP-1 were observed. Results： The degrees of pathological lesion in group C was less than that of group B （P〈0.05） . The expression of MMP-1 in group C was lower than that of group B （P〈0.01）, and the expression of TIMP-1 in group C was higher than that of group B （P〈0.01） . Conclusion： Musk-Aconite-Earthworm pill can lessen cartilage cataplasia. The mechanism is possibly related to the regulation on MMP-1 and TIMP-1.

关 键 词：麝香乌龙丸 膝骨关节炎 基质金属蛋白酶 基质金属蛋白酶抑制剂 

分 类 号：R258.5[医药卫生—中医内科学]