水溶性CdTe量子点的合成及其用于荧光猝灭法测定肌红蛋白  

Synthesis of water-soluble CdTe quantum dots and the detection of myoglobin based on its fluorescence quenching to CdTe quantum dots

作　　者：闫炜[1] 张爱梅[1] 梅艳丽[1] 李红芳[1] 王怀生[1,2] 

机构地区：[1]聊城大学化学化工学院,聊城252059 [2]聊城大学材料科学与工程学院,聊城252059

出　　处：《分析试验室》2008年第12期1-5,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20475024;20775031);聊城大学科研基金(X061003)项目资助

摘　　要：以3-巯基丙酸为稳定剂，合成了具有特殊光学性质的水溶性CdTe量子点，其最大发射波长位于544nm。利用荧光光谱、紫外可见光谱及圆二色光谱法系统的研究了CdTe量子点与肌红蛋白（Mb）二者结合前后体系光谱的变化，从而证实了CdTe量子点与Mb之间静电结合反应的特征。在pH7．0的PBS缓冲液中，用CdTe量子点作为荧光探针研究了肌红蛋白与量子点的相互作用，并基于肌红蛋白对CdTe量子点有显著的荧光猝灭作用，建立了肌红蛋白的快速检测方法。在最佳实验条件下，该体系荧光强度的猝灭程度（AF）与肌红蛋白质量浓度呈良好的线性关系，线性范围为0．3～24ug／mL，检出限为0．13ug／mL。该方法已对合成样品中肌红蛋白进行检测，并用于人体尿样中肌红蛋白的测定。The water-soluble CdTe quantum dots which have spectral properties were synthesized and modified by using 3-mereaptopropionic acid in aqueous solution. The maximum emission wavelength of CdTe QDs is at 544 nm. The interactions of CdTe with myoglobin were studied in pH 7.0 PBS buffer solution, and a rapid detection method of Mb was established based on the fluorescence quenching of myoglobin to the CdTe QDs, using CdTe QDs as a fluorescence probe. Under optimum conditions, the extent of the fluorescence quenching was linearly proportional to the concentration of Mb from 0.3 to 24ug/mL, with a detection limit of 0.13ug/mL. This method is simple, rapid, sensitive and shows promise application to the detection of Mb in the biologic systems and urine samples.

关 键 词：CDTE量子点 肌红蛋白 荧光猝灭 荧光探针 

分 类 号：O557.3[理学—热学与物质分子运动论]