液相微萃取-反萃取在磺胺药物残留测定中的应用  

Application of liquid-phase micro-extraction with back extraction in determination of sulfonamide residues in animal tissues

作　　者：陈璇[1] 白小红[1] 冯婷[1] 王晓园[1] 

机构地区：[1]山西医科大学药学院,太原030001

出　　处：《分析试验室》2008年第12期47-50,共4页Chinese Journal of Analysis Laboratory

基　　金：山西省自然科学基金(2007011086)项目资助

摘　　要：利用液相微萃取-反萃取技术，建立测定动物组织中磺胺药物残留量的新方法。将动物组织中磺胺药物经液相微萃取-反萃取后进行高效液相色谱紫外检测。磺胺、磺胺嘧啶、磺胺二甲嘧啶、磺胺喹噁啉4种磺胺类药物的线性范围均在0．05～10．0ug/mL，磺胺甲噁唑的线性范围在0．5～10．0ug／mL之间．方法的平均回收率不低于80．2％，RSD〈6．8％，检出限为0．1mg／kg。A new method for the determination of sulfonamide residues in animal tissues by the liquid-phase micro-extraction with back extraction technology is developed. The sulfonamides in animal tissues were extracted and purified, and then the extracted solution was determined by high performance liquid chromatography with a ultraviolet detector. The linear ranges of Sulfa, SD, SM2, SQ were 0.05 - 10.0 ug/mL, The linear range of SMZ was 0.5 - 10.0 ug/mL. The average recovery was higher than 80.2%. RSD was lower than 6.8%. The detection limit was 0.1 mg/kg. The method is accurate, effective, and fast, and it has been applied to the determination of sulfonamides residues in animal tissues with satisfactory results.

关 键 词：液相微萃取-反萃取 高效液相色谱 动物组织 磺胺残留 

分 类 号：O657.7[理学—分析化学]