Ca^(2+)对高温强光胁迫下小麦叶片光合机构的保护作用  

Protective Effect of Ca^(2+) on Photosynthetic Apparatus of Wheat Leaves under Heat and High Irradiance Stress

作　　者：杨亚军[1] 李利红[2] 赵会杰[3] 马培芳[3] 张超男[1] 

机构地区：[1]河南农业大学资源与环境学院,河南郑州450002 [2]郑州牧业工程高等专科学校,河南郑州450002 [3]河南农业大学生命科学学院,河南郑州450002

出　　处：《麦类作物学报》2008年第6期1042-1047,共6页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(30671214)

摘　　要：为了研究Ca2+对高温强光胁迫下小麦光合机构的保护机理,分别用清水和10 mmol/L的CaCl2水溶液预处理灌浆期的小麦叶片,然后进行不同的温度和照光处理,探讨了Ca2+对高温强光胁迫下小麦叶片D1蛋白和PSⅡ功能的影响。结果表明,叶面喷施Ca2+有效地调节了高温强光下抗氧化酶SOD、APX的活性,降低了叶片MDA的含量和相对电导率;Ca2+处理维持了较高的最大光化学效率(Fv/Fm)和较低的原初荧光(Fo),减轻了逆境对光合机构的破坏。Western Blotting分析结果也显示,Ca2+减轻了高温强光对小麦叶片PSⅡ反应中心D1蛋白的破坏。The protective mechanism of Ca^2＋ on photosynthetic apparatus of wheat leaves under heat and high irradiance stress were investigated. The leaves of wheat were sprayed by solution of CaCl2 （10 mmol/L） or water （as control） at grain-filling stage and exposed to different temperature and irradiance. The effect of Ca^2＋ on D1 proteins and photosystem Ⅱ（PSⅡ） performance of wheat leaves under heat and high irradiance stress were studied. The results showed that pretreatment with Ca^2＋ enabled the wheat leaves to keep relatively high activities of superoxide dismutase （SOD） and ascorbate peroxidase （APX）, lower malondialdehyde （ MDA ） content and relative electrical conductivity,higher Fv/Fm and lower Fo. The analysis of Westen Blotting indicated that Ca^2＋ pretreatment can decrease the degradation of D1 proteins.

关 键 词：小麦 钙离子 高温强光胁迫 抗氧化酶 D1蛋白 

分 类 号：S512.1[农业科学—作物学] S311