瘢痕疙瘩临床表型与p53基因72位编码子基因多态性的相关性分析  

CORRELATION ANALYSIS BETWEEN CLINICAL PHENOTYPES OF KELOIDS AND POLYMORPHISM OF p53 GENE CODON 72

作　　者：刘永波[1,2] 高建华[1] 刘晓军[1] 鲁峰[1] 刘宏伟 

机构地区：[1]南方医科大学南方医院整形外科,广州510515 [2]广州军区总医院整形外科 [3]暨南大学华侨医院整形外科

出　　处：《中国修复重建外科杂志》2008年第12期1433-1436,共4页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的观察并探讨p53基因表达及其72位编码子基因多态性对瘢痕疙瘩临床表型的影响。方法取35例自愿捐献瘢痕疙瘩组织,男19例,女16例;病程4个月～8年。同时收集自身外周血作基因型分析。根据观察范围不同,实验共分为两组(n=35)。中央组:各瘢痕疙瘩中央部(中央2/3半径范围内);周边组:各瘢痕疙瘩周边部(周边1/3半径范围内)。两组再根据瘢痕疙瘩最大横径大小分3个亚组:<1cm为小体积组(n=5),1～3cm为中体积组(n=21),>3cm为大体积组(n=9)。采用PCR法扩增p53基因外显子4,并进行基因测序;免疫组织化学方法检测P53蛋白表达变化;TUNEL法检测瘢痕疙瘩组织中成纤维细胞凋亡,并进行统计学分析。结果瘢痕疙瘩p53遗传基因型为Arg/Arg者7例,Pro/Arg者21例,Pro/Pro者7例,与临床表型分布有相关性(P<0.05);中、小体积周边组及中央组P53蛋白染色阳性,大体积周边组偶见细胞染色阳性。基因型为Arg/Arg、Arg/Pro及Pro/Pro的标本吸光度值分别为3439.3598±538.5275、3273.1862±375.2139及1691.3729±98.9893,各基因型间比较,差异有统计学意义(P<0.05);TUNEL法检测成纤维细胞凋亡,镜下可见凋亡细胞呈均匀分布,大、中、小体积周边组凋亡细胞指数分别为31.0000±3.2660、42.3000±4.3548、44.6000±5.2536,各组间比较差异有统计学意义(P<0.05)。结论瘢痕疙瘩临床表型与P53蛋白表达密切相关,组织中p53基因72位编码子基因多态性的变异有利于瘢痕疙瘩成纤维细胞凋亡。Objective To observe the effect of gene expression of p53 and the polymorphism of p53 gene codon 72 on clinical phenotype of keloids. Methods The tissue and blood samples were taken from 35 patients with keloids, 19 males and 16 females, and the course of disease was from 4 months to 8 years. Meanwhile, autologous peripheral blood was collected for genotype analysis. According to the observing scope, the tissue samples of the keloids were divided into 2 groups： the central group involving the central part of the keloids （the central area within two-thirds of the radius） and the peripheral group involving the peripheral part of the keloids （the peripheral area within one-third of the radius）. According to the largest diameter of the keloids, the two groups were divided into 3 subgroups： the small size group with 5 patients （〈 1 cm）, the medium size group with 21 patients （1-3 cm） and the large size group with 9 patients （〉 3 cm）. DNA of the tissue and blood samples were extracted, and the PCR followed by DNA sequencing was used to detect the polymorphism of p53 gene codon 72. The expression change of P53 was detected by immunohistochemical staining. The fibroblast apoptosis in keloid tissues was detected by TUNEL method. Results The genetic genotype of p53 gene codon 72 in keloids included Arg/Arg in 7 cases, Pro/Arg in 21 cases, Pro/ Pro in 7 cases. The significant correlation was found between genotype and clinical phenotype （P 〈 0.05）. Immunohistochemical staining revealed that P53 was detectable in peripheral and central groups of small-medium size keloids and central groups keloids, and detectable in few cells in peripheral groups of large size keloids. The absorbency value was 3 439.359 8 ± 538.527 5 in Arg/Arg genotype, 3 273.186 2 ± 375.213 9 in Arg/Pro genotype, 1 691.372 9 ± 98.989 3 in Pro/Pro genotype. There were significant differences among the three genotypes （P 〈 0.05）. The fibroblast apoptosis was detected by TUNEL, and the apoptotic cells were evenly dis

关 键 词：瘢痕疙瘩 P53蛋白 基因多态性 成纤维细胞 

分 类 号：R622[医药卫生—整形外科]