皮肤毛细血管瘤模型鸡冠的激光和光动力学治疗研究  

RESEARCH ON LASER AND PHOTODYNAMIC THERAPY OF SKIN HEMANGIOMA MODEL-COMB

作　　者：廖明德[1] 赵劲民[2] 陈石海[1] 韦强[1] 殷国前[1] 刘庆丰[1] 

机构地区：[1]广西医科大学第一附属医院整形美容外科,南宁530021 [2]广西医科大学第一附属医院创伤骨科,南宁530021

出　　处：《中国修复重建外科杂志》2008年第12期1437-1440,共4页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的进一步探索毛细血管瘤的激光和光动力学疗法(photodynamic therapy,PDT)治疗机制。方法取6月龄雄性莱亨鸡48只,随机分为6组,每组8只。鸡冠未处理侧作为自身对照。A组:不作任何处理;B、C组:分别以能量密度10J/cm2和15J/cm2激光照射鸡冠1次,持续2～3min;D组:于左腋下静脉注射血啉甲醚(hematoporphyrin monomethyl ether,HMME)10mg/kg1次;E、F组:同D组注射HMME后10min,分别以能量密度为10J/cm2和15J/cm2激光照射鸡冠。观察处理后动物一般情况,并于处理后14d取各组鸡冠组织块行HE染色观察,计算毛细血管减少率,并行TUNEL染色观察血管内皮细胞凋亡情况。结果术后动物一般情况良好,无死亡,A组及D组鸡冠外形及色泽无明显变化,其余各组出现灰白斑,组织水肿、坏死和黑痂。组织学观察A、D组鸡冠无明显变化,其余各组可见组织液渗出、水肿、炎性细胞浸润、血管数目减少、血管管径变小及管腔内血栓形成,部分血管内皮细胞凋亡。A～F组毛细血管减少率分别为7.87%±2.50%、48.58%±5.10%、57.58%±8.17%、8.84%±3.13%、61.26%±16.87%、68.56%±10.54%;凋亡指数分别为1.05±0.76、82.22±6.18、94.13±1.64、1.38±0.52、101.88±5.94、104.88±6.22;B、C、E、F组两项指标与A、D组比较差异均有统计学意义(P<0.01),B、E组间及C、F组间比较差异有统计学意义(P<0.01),B、C组间和E、F组间比较差异均无统计学意义(P>0.05)。结论激光及PDT治疗毛细血管瘤除了直接封闭血管外,通过诱导血管内皮细胞凋亡也可能是其治疗机制之一。Objective To explore the mechanism of laser and photodynamic therapy （PDT） of hemangioma Methods A total of 48 6-month-old male chickens were divided into 6 groups, with 8 in each group. The side with the untreated comb served as the control. Group A was the control group. In groups B and C, the combs received laser irradiation once of 10 J/cm^2 and 15 J/cm^2, respectively, for 2-3 minutes. In group D, hematoporphyrin monomethyl ether （HMME） of 10 mg/kg was injected into the left-side axillary vein once. In groups E and F, the combs received laser irradiation of 10 J/cm^2 and 15 J/cm^2, respectively, after 10 mg/kg HMME was injected for 10 minutes into the left-side axillary vein. On the 14th day, HE staining was performed and the decrease rate of the number of capillary vessels was calculated. Besides, TUNEL staining was conducted to observe the condition of capillary endothelium apoptosis. Results No animal died after the operation, the combs in groups A and D had no obvious changes in shape and color, but the ones in the other groups had leukasmus, edema, necrosis and dark crust. According to histological observation, in groups A and D, the combs had no obvious changes. But in the other groups, tissue exudation, edema, inflammatory cell exudation, decreasing number of vessels, waning vascular caliber, thrombus, and partial capillary endothelium apoptosis were found. The decrease rate of the number of capillary vessels was as follows： 7.87% ± 2.50% in group A, 48.58% ± 5.10% in group B, 57.58% ± 8.17% in group C, 8.84% ± 3.13% in group D, 61.26% ± 16.87% in group E and 68.56% ± 10.54% in group F. The apoptosis index in all groups was 1.05 ± 0.76, 82.22 ± 6.18, 94.13 ±1.64, 1.38 ± 0.52, 101.88 ± 5.94, 104.88 ± 6.22, respectively. There were significant differences between groups B, C, E, F and groups A, D in the above two items （P 〈 0.01）. There were significant differences between groups B and E as well as between groups C and F （P 〈 0.01）. There were no significant differences

关 键 词：毛细血管瘤 光动力学疗法 血管内皮细胞 凋亡 

分 类 号：R622[医药卫生—整形外科] R616.4[医药卫生—外科学]