一步法RT-PCR检测人狂犬病毒初探  

Primary application of one-step RT-PCR in detection of human rabies

作　　者：孙来晶[1,2] 王华林[2] 徐葛林[3] 吴杰[3] 胡志红[2] 周亦武[1] 

机构地区：[1]华中科技大学同济医学院法医学系,湖北武汉430030 [2]中国科学院武汉病毒研究所病毒学国家重点实验室,湖北武汉430074 [3]中国生物制品集团公司武汉生物制品研究所基因工程室,湖北武汉430070

出　　处：《中国法医学杂志》2008年第6期400-402,共3页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30570629)

摘　　要：目的探讨一步法逆转录聚合酶链反应(one-step reverse transcriptase-PCR或一步法RT-PCR)在人狂犬病检测中的应用。方法4例临床诊断狂犬病死亡患者的冰冻脑组织,用一步法RT-PCR检测病毒,狂犬病毒CVS株感染的鼠脑和急性胰腺炎死者的脑分别作为阳性和阴性对照。结果4例脑组织中提取的RNA,电泳后得到与狂犬病N基因长度一致的片断。结论一步法RT-PCR可用于人狂犬病毒的检测,在法医学鉴定中具有重要意义。Objective To discuss the application PCR） in the detection of human rabies. Methods The of one-step RT-PCR（one-step reverse transcriptasefreezing identified by clinical diagnosis were collected and were detected brain tissues of four patients died of rabies by one-step RT-PCR. The rat brain infected by CVS strain of rabies virus was used as a positive control. The brain of a patient died of acute pancreatitis was used as a negative control. Results The RNA could be extracted from the freezing tissues. After one-step RT-PCR, specific bands with the expected size of the N gene could be detected in the samples from all the 4 cases. Conclusion One-step RT-PCR could be used in detection of human rabies. It was important for forensic identification.

关 键 词：法医物证学 人狂犬病 一步法RT—PCR 法医学鉴定 

分 类 号：D919[医药卫生—法医学]