急性心肌梗死急诊PCI术后无复流与NF-κB、AP-1和GR水平关系的研究  

Relationship between change of the nuclear protein level of NF-κB,AP-1 and GR and no-reflow in patients with acute myocardial infarction

作　　者：任法新[1] 方毅民[1] 杨军[1] 刘少荣[1] 张传焕[1] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院心内科,山东省烟台市264000

出　　处：《中国心血管病研究》2008年第12期885-887,共3页Chinese Journal of Cardiovascular Research

基　　金：山东省烟台市科学技术发展计划资助项目(2005126)

摘　　要：目的探讨核转录因子核因子-κB(NF-κB)、活化蛋白-1(AP-1)和糖皮质激素受体(GR)的核内蛋白水平变化与心肌无复流现象(NR)的关系。方法26例行急诊经皮冠脉介入治疗(PCI)的ST段抬高型心肌梗死(STEMI)患者,经单光子发射型计算机断层(SPECT)心肌灌注显像检测,出现心肌无复流现象。采用WesternBlott法检测STEMI急诊PCI术后无复流患者外周血单个核细胞(PBMC)中NF-κB、AP-1和GR的核内蛋白水平的变化及这种变化与NR的关系。结果无复流组患者NF-κBp65、c-Jun/AP-1的蛋白表达水平显著高于复流组(P<0.05);无复流组患者GR-α的蛋白表达水平显著低于复流组(P<0.05)。结论急性心肌梗死(AMI)急诊PCI术后出现心肌无复流患者存在NF-κB、AP-1的激活和GR的抑制。Objective To explore the relationship of the change of the nuclear protein level of nuclear factor-kappa B （NF-KB）, activator protein-1 （AP-1） and glucocorticoid receptor （GR） and no-reflow （NR） after treatment with emergency percutaneous coronary intervention（PCI） in patients with ST-elevant acute myocardial infarction （STEMI）. Methods 26 patients observed NR by single photon emission computed tomography（SPECT） after treatment with emergency PCI in STEMI patients. The nuclear protein level of activated NF-κB, AP-1 and GR were detected by Western Blotting assay, and demi-quantity image analysis was done. Results The protein expression of NF-κB p65,c-Jun/AP-1 in mononuclear cells in NR group was significantly higher than that in reflow group （P〈0.05）. The protein expression of GR-α in mononuelear cells in NR group was significantly lower than that in reflow group（P〈0.05）. Condusion Patients with no-reflow have the activation of NF-κB p65 ,c-Jun and the inhibition of GR in peripheral blood mononuclear celI（PBMC）.

关 键 词：心肌梗死 糖皮质激素受体 活化蛋白-1 核因子-κB 无复流 

分 类 号：R542.2[医药卫生—心血管疾病]