糖尿病创面愈合与粒细胞巨噬细胞集落刺激因子关系的研究  

To study the relationship between wound healing of diabetesmellitus andgranulocyte-macrophage colony-stimulating factor

作　　者：李斌[1] 吴晓勇[1] 徐丽红[1] 黄伟琪[1] 王健[1] 邓海涛[1] 沈耀明[1] 方勇[2] 

机构地区：[1]东南大学附属江阴医院整形烧伤科,江苏省江阴市214400 [2]上海交通大学医学院第三人民医院烧伤整形科

出　　处：《中国现代医药杂志》2008年第11期24-26,共3页Modern Medicine Journal of China

基　　金：东南大学附属江阴医院立项课题。

摘　　要：目的探讨糖尿病创面愈合与粒细胞巨噬细胞集落刺激因子的关系。方法70只C57BL/6小鼠分为野生小鼠组(对照组,n=35)和糖尿病模型组(DM组,n=35)。腹腔麻醉后在背部中线两侧各制作0.8cm×0.8cm创面。创面动态摄像并于相应时间段取标本,观察创面组织愈合情况,同时计算创面愈合率;ELISA法测定创面GM-CSF表达,显微镜下观察各组创面免疫细胞数目。结果创面形成后第3天起,DM组小鼠创面愈合率较对照组明显下降,以创面形成后7d内变化最为明显;创面形成后第1天,两组小鼠创面GM-CSF表达均明显增高;创面形成后第1天和第3天,对照组小鼠创面GM-CSF表达显著高于DM组。2组动物在伤后第5天,T淋巴细胞均达到峰值,DM组显著低于对照组,7～14天DM组稍高于对照组。结论GM-CSF的低表达则可能与创面愈合早期炎症细胞浸润减少有关。Objective To study the relationship between wound healing of diabetes mellitus and granulocyte -macrophage colony-stimulating factor.Methods Seventy C57BL /6 mice were obtained and divided into control group（n= 35） and DM group（n = 35）.All mice were received full thickness skin wound（0.8cm ×0.8cm） in each side of midline after deep anesthesia.The wound sites were digitally photographed to calculate the percentage of wound closure using computer image analysis software in different time points post -injury.The content of GM-CSF in the wound sample was measured by ELISA.Results The analysis of wound closure showed that wound healing in DM mice was significantly delayed compared with that in the control mice from the third day post-injury.In the DM mice,the levels of GM-CSF expression were increased markedly from the first day post-injury and declined to lower levels in the two groups.At the first day and third day post -injury,GM-CSF levels in DM mice were significantly lower than those in the control mice.Conclusion The delayed wound closure in DM mice may be lower expression of GM-CSF in wound site.

关 键 词：糖尿病 创面愈合 粒-巨噬细胞集落刺激因子 

分 类 号：R587.2[医药卫生—内分泌]