马尾松银松素合酶基因启动子区的克隆及特征分析  

Molecular cloning and analysis of pinosylvin synthase gene promoter from Pinus massoniana

作　　者：王冰梅[1,2,3] 郭晋隆[2] 叶冰莹[1,2,3] 许莉萍[2] 陈由强[1,2,3] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108 [2]农业部甘蔗遗传改良重点开放实验室,福建福州350002 [3]福建师范大学发育与神经生物学福建省高等学校重点实验室,福建福州350108

出　　处：《亚热带农业研究》2008年第4期292-296,共5页Subtropical Agriculture Research

基　　金：国家自然科学基金(30371177);福建省教育厅基金(JA07045)资助项目

摘　　要：银松素合酶(pinosylvin synthase,PS)是合成银松素类植物抗毒素的关键酶。本文根据前期工作所获得的PS基因序列,设计基因特异引物,利用连接介导的染色体步行技术,从马尾松总基因组中扩增PS基因上游的启动子区;通过PLAN-CARE等分析软件对5′侧翼区近700 bp进行启动子特征分析,预测启动子区调控元件。结果表明,在翻译起始密码子上游167 nt处可能存在TATA-box。此外还发现3个GATA-box(-260 nt、-295 nt、-386 nt和-295 nt)和若干个TGAC-like序列。Pinosylvin synthase （PS）, is a key enzyme in the biosynthesis of pinosylvin-type phytoalexins. In the study, PS gene specific primers were designed, and the upstream sequence of PS gene was cloned by genomic walking based on ligation-mediated PCR method. The regulatory element of gene promoter was analyzed by PLANCARE on line. This sequence was about 700 bp in length, containing one TATA-box, three GATA-boxes, and several TGAC-like sequences.

关 键 词：银松素合酶 基因克隆 染色体步行 启动子分析 

分 类 号：Q946[生物学—植物学]