大鼠脊髓损伤后运动终板内乙酰胆碱酯酶和降钙素基因相关肽的变化  

Changes of Calcitonin Gene-related Peptide and Acetylcholine Esterase in Motor End Plates after Spinal Cord Injury in Adult Rats

作　　者：张皑峰[1] 欧喜超[2] 杨朝阳[2] 刘玉军[2] 鲁强[2] 李晓光[2] 

机构地区：[1]首都医科大学附属友谊医院口腔科,北京市100050 [2]首都医科大学北京神经科学研究所,北京市神经再生修复研究重点实验室,教育部神经变性病学重点实验室,北京市100069

出　　处：《中国康复理论与实践》2008年第11期1030-1032,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：北京市自然科学基金项目(7041002);北京市教育委员会科技发展计划项目(KZ200310025008)

摘　　要：目的观察大鼠不同类型脊髓损伤后降钙素基因相关肽(CGRP)与乙酰胆碱酯酶(AChE)在后肢腓肠肌运动终板内的变化,以探讨不同类型脊髓损伤后CGRP和AChE与运动终板退变的关系。方法60只成年Wistar大鼠随机分为假手术组(A组)、切割型脊髓损伤组(B组)和撞击型脊髓损伤组(C组),术后1、2、4、10周分别用Karnovsky-Roots直接法染色检测AChE、免疫组化法检测CGRP的变化。结果两损伤组大鼠术后运动终板中CGRP和AChE浓度均下降,但C组10周时有了明显恢复,而B组一直处于较低水平;CGRP的改变早于AChE。结论在大鼠不同类型脊髓损伤后,CGRP和AChE的活性和浓度与运动终板退变的程度不同。Objective To explore the degeneration of motor end plates （MEP） by observing the expression of calcitonin gene-relative peptide （CGRP） and acetylcholine esterase （ACHE） in the MEP after different types of spinal cord injury. Methods 60 adult female Wistar rats were randomly assigned to 3 groups： sham group, completely transection group and contusion group. The content of AChE in the MEP was detected with Karnovsky Roots staining and the expression of CGRP was then determined with immunohistochemistry. Results The content of both AChE and CGRP significantly decreased after either type of spinal cord injury. However, their activity gradually recovered to the normal level in the contusion group, but not in the transection group. Moreover, the changes of CGRP occurred earlier than those of ACHE. Conclusion The motor end plate degenerates differently after different kinds of spinal cord injury in adult rat, CGRP and AChE are related to the degeneration of MEP.

关 键 词：脊髓损伤 运动终板 降钙素基因相关肽 乙酰胆碱酯酶 大鼠 

分 类 号：R651.2[医药卫生—外科学]