提取与分离方法影响海兔口腔神经节蛋白质组的分离效果  

Effect of Both Extraction and Separation on Separation Effect of Proteome from the Buccal Ganglion of Aplysia

作　　者：朱斌琳[1] 陈东仕[1] 翁露娜[1] 颜利[1] 黄河清[1] 

机构地区：[1]厦门大学生命科学学院,生物化学与生物技术学系,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2008年第A02期96-99,共4页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(40776060)资助

摘　　要：选用丙酮沉淀法和直接裂解法分别提取离蓝斑背肛海兔（Notarcus leachii cinvsus Stimpson，NLCS）口腔神经节（Buccal Ganglion，BG）全蛋白质，发现采用直接裂解法所获得BG蛋白质种类多于丙酮沉淀法．在第一向等电聚焦电泳（IEF）中，分别选用pH为3．0—9．0，4．0～6．0和5．0～8．0的载体两性电解质，发现载体两性电解质pH范围为5．0—8．0时分离效果最好．使用Melanie 4 Trial软件统计BG蛋白质斑点数目约为290点，适于开展BG蛋白质组学研究．The total proteins ofbuccal ganglion from Aplysia were extracted by acetone precipitation or direct lysis. The results showed that more proteins could be obtained on the 2D-PAGE map by the latter approach. The carrier ampholytes of pH range 3.0 -9.0, 4.0 - 6.0, and 5.0 -8.0 were used in IEF respectively, it showed that the best separation effect could be obtained in pH range of 5. 0-8.0. And there are approximately 290 spots in the gel, these perfect datum indicated that this approach described here was suit for doing research about pro- teomics.

关 键 词：蓝斑背肛海兔 蛋白质组 PH 优化提取与分离 

分 类 号：Q51[生物学—生物化学]