羧基微珠表面化学与生物活化方法及表征  

Chemo-/Bio-Activation and Characterization on Surface of Carboxyl Microbeads

作　　者：陈雪[1] 周雷激[1] 

机构地区：[1]厦门大学化学化工学院化学系,现代分析科学教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2008年第A02期201-203,共3页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(20775065;20835005);教育部高校博士点基金(20070384023);化学生物传感与计量学国家重点实验室(湖南大学)开放课题(2006021);国家基础科学人才培养基金(J0630429)资助

摘　　要：研究了微珠阵列芯片传感单元的生物分子固定化方法.运用羰基二咪唑(CD I)法进行羧基微珠表面化学活化研究及表征.结果表明微珠表面咪唑基团的有效活化率为30.1%.活化微珠表面固定化抗体后,采用荧光显微成像分析,CD I法活化的微珠比未活化的微珠荧光强度提高60倍,活化效果显著.An approach of bio-molecular immobilization on the surface of the carboxyl microbeads, the sensing units of the microbead arrays, is presented. In this method, carboxyl microbeads were activated using N, N-Carbonyldiimidazole （CDI）, and the activated groups and the activation efficiency were quantitatively measured and calculated. As a result of the immobilization of the antibodies on the activated microbeads and the fluorescence microscopic imaging analysis, the fluorescence signal of the activated beads is 60-fold higher than that of the unactivated beads, which shows high activation efficiency.

关 键 词：羧基微珠 固定化 荧光 生物芯片 

分 类 号：O65[理学—分析化学]