基于氧猝灭原理的淡水BOD微生物传感器  

BOD Sensing Film Based on Fluorescence Quenching by Oxygen in Freshwater

作　　者：周廷尧[1] 王旭东[1] 赵赟[1] 陈海旭[1] 陈曦[1,2] 

机构地区：[1]厦门大学化学化工学院,现代分析科学教育部重点实验室,福建厦门361005 [2]近海海洋环境科学国家重点实验室(厦门大学),福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2008年第A02期208-212,共5页Journal of Xiamen University：Natural Science

基　　金：国家基础科学人才培养基金(J0630429);福建省发展与改革委员会“小发明、小创造”专项资助

摘　　要：本研究使用溶胶-凝胶法固定二高氯酸4，7-二苯基-1，10-邻菲咯啉钉[Ru（app），（ClO4）2]作为氧敏感材料，采用溶胶嵌胶杂化聚乙烯醇（PVA）法包埋枯草芽孢杆菌和地衣芽孢杆菌耐得测量淡水样品的BOD微生物传感膜．在此基础上，考察了传感膜的响应时间、线性、稳定性以及温度、pH的影响，结果表明制得的BOD微生物传感器在2—30mg／t范围内具有良好的线性和重现性．实验还初步尝试使用自制的BOD微生物传感器对地表水样品BOD进行检测．A sensing film for biochemical oxygen demand （BOD） in freshwater was developed, which consisted an organically-modified silicate （ORMOSIL） film embedded with tri（4,7-diphenyl-1,10-phenanthroline） ruthenium（Ⅱ） perchlorate as oxygen indicator and sol-gel hybrid PVA matrix immobilized with aerobes, Bacillus subtilis and Bacillus licheniformis respectively. A series of experiments for characterizing the sensor behaviors were carried out including response time, reproducibility, linear range et al. Effects of temperature and pH on the BOD sensing films were studied as well. Results showed that the sensing film had a good linear range in 2 -30 mg/L and a good reproducibility （RSD ≤4.0 % ）. Primary determination of BOD in freshwater was also carried out.

关 键 词：淡水 BOD 微生物传感膜 溶胶-凝胶 

分 类 号：O657.3[理学—分析化学]