兴安升麻总苷抗肿瘤药效研究  

Experimental Study on Antitumor Effects of Total Glycoside of Cimicifuga dahurica Maxim.

作　　者：曹丽[1] 杨卫彬[2] 潘瑞乐[1] 李玲玲[1] 金文[1] 孙虹[1] 孙晓波[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100193 [2]中国中医科学院基础理论研究所,北京100700

出　　处：《中国中医药信息杂志》2008年第12期31-33,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家科技部"十五"重大科技攻关项目(2001BA701A55-13)

摘　　要：目的观察升麻总苷对小鼠移植性肿瘤和人肿瘤细胞裸鼠移植瘤的体内抗肿瘤作用,以及体外对人肿瘤细胞株的抑瘤活性,并对其抑瘤机制进行初步探讨。方法MTT法检测体外升麻总苷对人肿瘤细胞的增殖抑制作用;体内实验观察其对小鼠S180和裸鼠体内移植人肺腺癌A549的抑制作用;同时采用流式细胞仪和肿瘤病理切片对接种的A549肿瘤细胞凋亡进行观察。结果升麻总苷对A549、HepG2、HL60、Eca-109、MDA-MB231肿瘤细胞的半数抑制浓度(IC50)分别为20.3、27.1、21.2、23.4和32.7μg/mL。小鼠口服升麻总苷100mg/kg和200mg/kg可明显抑制S180移植瘤(抑瘤率分别为42.8%和54.6%)和裸鼠移植人肺腺癌A549的生长(T/C值分别为58.1%和52.2%),肿瘤组织病理切片和流式细胞仪对A549肿瘤细胞凋亡的检测显示,升麻提取物可诱导体内肿瘤细胞凋亡。结论升麻体内体外均具有抗肿瘤活性,其体内抑制肿瘤生长可能与诱导细胞凋亡相关。Objective To investigate the antitumor effects of total glycoside of Cimicifuga dahurica （TGCD） in vivo and in vitro, and further explore its mechanisms. Methods The anti-tumor activity in vitro was determined by MTT assay and the anti-tumor activity in vivo was evaluated using experimental mouse tumor （S180） model and human tumor （A549） xenografts in nude mice. After treatment, A549 cell apoptosis and morphologlc change were evaluated by Annexin V/PI flow cytometry and HE staining. Results Inhibitory concentration 50% （IC50） of TGCD on A549, HepG2, HL60, Eca-109 and MDA-MB231 cells were 20.3, 27.1, 21.2, 23.4 and 32.7 μg/mL respectively. Administration of TGCD （100 or 200 mg/kg） inhibited S180 solid tumor development in mice, the inhibition rates were 42.8% and 54.6% respectively. Administration of TGCD （100 or 200 mg/kg） inhibited A549 tumor growth with a T/C （mean value of treated group/mean value of control group） value of 58.1% and 52.2% respectively. In addition, increased percentage of apoptotic cells induced by TGCD in human A549 nude mice xenografts and the histopathological changes including cell shrinkage and condensation of chromosomes were observed. Conclusion TGCD has demonstrated antitumor bioactivity both in vitro and in vivo, which may be related to its effects of inducing apoptosis activity.

关 键 词：升麻总苷 小鼠S180肉瘤 裸鼠 人肺腺癌A549 凋亡 

分 类 号：R285.5[医药卫生—中药学]