南海海域几丁质降解菌的筛选及其特性研究  

Isolation and Characterization of Chitin-degrading Bacteria from South China Sea

作　　者：蔡亚萍[1] 苏建强[1] 谢忠[1] 郑天凌[1,2] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005 [2]近海海洋环境科学国家重点实验室(厦门大学),福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2008年第A02期259-263,共5页Journal of Xiamen University：Natural Science

基　　金：国家863计划(2008AA09Z408);国家自然科学基金(40876061);中国博士后科学基金(20070420747);国家基础科学人才培养基金项目(J0630649)资助

摘　　要：从南海海域水样和沉积物中分离并筛选到两株几丁质降解菌，分别为SCSS04和SCSWE13．16SrDNA序列分析结果表明，菌株SCSS04属于芽孢杆菌属，菌株SCSWE13属于弧菌属．两株菌几丁质酶的产生均需要几丁质的诱导，并在富营养培养基中产酶量较高．菌株SCSS04在培养第9天产酶量达到最高，所产生的几丁质酶最适反应温度为40—50℃．菌株SCSWE13属于低温酶，在培养第7天产酶量达到最高，所产生的几丁质酶最适反应温度为20-28℃．In this study, 2 strains of chitin-degrading bacteria were screened from the water sample and sediment of South China Sea, namely SCSS04 and SCSWE13. Sequence analysis of 16S rDNA showed that strain SCSS04 belonged to genus Bacillus and strain SCSWE13 belonged to genus Vibtio. Presence of chitin was required for the production of chitinase, and high level of chinase activity occured in eutrophic medium. The chitinase producted by strain SCSS04 was most active at 40 - 50℃ and peaked at 9^th day. The chitinase producted by strain SCSWE13 belonged to cold active enzyme as it was most active at 20 -28℃, and peaked at 7^th day in batch cultivation.

关 键 词：南海 几丁质降解菌 几丁质酶 特性 

分 类 号：P745[天文地球—海洋生物学]