大鼠创伤性脑损伤后认知行为的变化  

The cognitive function changes after traumatic brain injury in rats

作　　者：苏月伟[1] 仲苏玉[2] 莫林宏[1] 刘玉军[1] 张皑峰[3] 杨朝阳[2] 李晓光[1,2] 

机构地区：[1]首都医科大学北京神经科学研究所北京神经再生修复研究重点实验室教育部神经变性病学重点实验室,北京100069 [2]北京航空航天大学 [3]首都医科大学附属北京友谊医院

出　　处：《山东医药》2008年第37期6-8,共3页Shandong Medical Journal

基　　金：北京市自然科学基金项目(7041002);北京市教育委员会科技发展计划项目(KZ200310025008)

摘　　要：目的建立直接手术损伤大鼠部分脑皮质及海马CA1区的创伤性脑损伤模型，并观察损伤后动物认知行为的改变情况。方法将大鼠进行脑损伤建模后，于术后11—15d和26—30d采用Morris水迷宫的方法评价动物的认知行为变化后，灌杀动物取脑切片，HE染色及尼氏染色观察损伤范围；胶质纤维酸性蛋白（GFAP）免疫组织化学染色观察星型胶质细胞（AST）的活化及胶质瘢痕形成情况；术后5d行Nestin及GFAP双重免疫荧光染色显示海马内干细胞的激活，并观察损伤边缘的干细胞迁移情况。结果直接手术损伤部分脑皮质及海马CA1区后，造成了动物认知功能障碍，并且1个月时有自发的认知功能恢复；5d时星型胶质细胞活化，1个月时胶质瘢痕的形成。结论本实验采用的模型可成功造成动物认知行为的改变，为组织工程材料的移植治疗脑损伤提供了比较适用的研究模型。Objective To establish traumatic brain injury model by removing part of brain cortex and region CA1 in hippocampus and to evaluate the cognitive function changes. Methods Wistar rat models with traumatic brain injury were established by surgery. After evaluated the cognitive function changes by Morris water maze, rats were perfused to get brain tissue. HE and Nissl＇s staining were employed. Activation of the astrocyte （AST） and glia scar formation were detected by immunohistochemistry of GFAP. Meanwhile the stem cells in hippocampus activation and migration to injury margin were detected by immunofluorescent double-labeling of Nestin and GFAP. Results The cognitive function deficit was made after removing part of cortex and region CA1 by surgery and there was cognitive recovery in 1 month; ASTs was activated when 5 days after surgery and glia scar was formed after 1 month. Conclusions The model of traumatic brain injury can make congnitive deficit, which offers a suitable model for the tissue engineering material transplantation treatment of brain injury.

关 键 词：创伤性脑损伤 海马CA1区 认知 大鼠 

分 类 号：R651.1[医药卫生—外科学]