HPLC法同时测定丹参类药材中水溶性活性成分的含量  

Simultaneous determination of water-soluble active components in Salvia miltiorrhiza Bge. and Salvia przewalskii Maxim. by HPLC

作　　者：杨树声 宋平顺[2] 赵建邦[2] 丁永辉 

机构地区：[1]定西市人民医院,甘肃定西743000 [2]甘肃省药品检验所甘肃省中药分析测试中心,甘肃兰州730000 [3]甘肃省食品药品监督管理局,甘肃兰州730000

出　　处：《甘肃中医学院学报》2008年第6期42-45,共4页Journal of Gansu College of Traditional Chinese Medicine

摘　　要：目的建立HPLC法同时测定丹参和甘肃丹参中丹参素钠、原儿茶醛和丹酚酸B含量的方法,通过3种水溶性活性成分测定,评价野生和甘肃栽培丹参、野生和栽培甘肃丹参的质量。方法色谱柱为C18色谱柱(4.6mm×250mm,5μm);流动相为甲醇—冰醋酸—水(20∶80∶1);流速1.0mL/min。二元梯度洗脱。结果丹参素钠、原儿茶醛、丹酚酸B分别在58～1455ng、15～162ng、825～8250ng范围内线性关系良好,回收率分别为102.20%、101.53%和103.03%。结论方法简便、准确、分离效果好,可作为丹参水溶性活性3种成分的同时定量测定方法。Objective To develop an HPLC method on the simultaneous determination of sodium danshensu, protocatechulaldehyde, salvianolic acid B in Salvia miltiorrhiza Bge. and Salvia przewalskii Maxim. Evaluated the quality of wild and cultivated Salvia miltiorrhiza Bge. and Salvia przewalskii Maxim. by determining three kinds of water-soluble active components in them. Methods Use the method dual gradient elution. The analysis was carried on a chromatographic C18（4.6 mm ×250 mm, 5 μm） with a mobile phase of methanol-glacial acetic acid-water（20： 80：1 ） at the flow rate of 1.0 mL/min. Results The linear correlation of sodium danshensu, protocatechuialdehyde, salvianolic acid B were well in 58 - 1455 ng, 15 - 162 ng and 825 - 8250 ng respectively. The average recoveries were 102.20 %, 101.53 % and 103.03 % respectively. Conclusion This method is convenient, accurate and rapid. It can be used to determine the three water-soluble active components in Salvia miltiorrhiza Bge. and Salvia przewalskii Maxim. at the same time.

关 键 词：高效液相色谱法 丹参 甘肃丹参 水溶性成分 丹参素钠 原儿茶醛 丹酚酸B 栽培 野生 

分 类 号：R284.1[医药卫生—中药学]