RP-HPLC法测定痛泻要方中芍药苷的含量  

RP-HPLC determination of Paeoniflorin in TongXieYaoFang

作　　者：赵辉[1] 方步武[2] 李玉仿 

机构地区：[1]天津市大港医院药剂科,天津300270 [2]天津医科大学药理教研室,天津300070 [3]天津市大港区药品检验所,天津300270

出　　处：《海峡药学》2008年第11期40-42,共3页Strait Pharmaceutical Journal

基　　金：天津市科技攻关计划重大科技项目(05YFGDSF02600)

摘　　要：目的建立反相高效液相色谱法测定痛泻要方中芍药苷的含量。方法采用Kromasil C18色谱柱（250mm×4．6mm,5μm）；以甲醇-水（25：75）为流动相；检测波长为230nm；流速为1．0ml·min^-1；柱温30℃。结果芍药苷在14．24～142．0μg·mL^-1范围内呈良好线性关系（r=0．99993，n=7）；平均回收率为100．07％，RSD为1．96％（n=6）。结论该方法测定结果灵敏、准确、重现性好，可用于痛泻要方中芍药苷的含量测定。OBJECTIVE To establish a reserved-phase HPLC method for the determination of Paeoniflorin in TongXieYaoFang. METHODS Kromasil ClS column （250mm×4.6mm, 5μm） was used with methanol-water （25：75） as the mobile phase, The detection wavelength was 230nm and the flow rate was 1.0mL· min^-1 with the column temperature of 30℃. RESULTS The linear response range was 14.24- 142.4μg·mL^- 1 （r = 0. 99993, n = 7） ; The average recovery rate was 100.07 %, RSD = 1.96 %. CONCLUSION The method was sensitive and accurate with good reproducibility. It is suitable for the studying on the content of Paeoniflorin in TongXieYaoFang.

关 键 词：反相高效液相色谱法 痛泻要方 芍药苷 含量测定 

分 类 号：R927.2[医药卫生—药学]