基质金属蛋白酶9和组织金属蛋白酶抑制剂1在大鼠肾脏机械性损伤后的表达  

Expressions of matrix metalloprotease-9 and tissue inhibitor of metalloproteinase-1 in kidneys of rats after mechanical injury

作　　者：李天民 刘忠[2] 毛渭东[3] 王兰鸣 庞华 

机构地区：[1]兰州市公安局七里河分局,甘肃兰州730050 [2]甘肃省中医院脊柱外科,甘肃兰州730050 [3]陕西省汉中市中心医院病理科,陕西汉中723000 [4]兰州市公安局西固分局,甘肃兰州730060

出　　处：《兰州大学学报（医学版）》2008年第4期15-17,23,共4页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的探讨大鼠肾脏机械性损伤后肾组织基质金属蛋白酶9(MMP-9)和组织金属蛋白酶抑制剂1(TIMP-1)的表达及意义。方法36只Wistar大鼠随机分为对照组和创伤组。采用自由落体生物撞击仪撞击大鼠脊肋角复制创伤动物模型。用组织芯片技术及免疫组织化学方法检测各组大鼠创伤后1、6、12、24和36h肾组织中MMP-9和TIMP-1蛋白的表达。结果对照组肾组织中MMP-9蛋白表达微弱,创伤组MMP-9表达在创伤后1h开始增强,6h迅速增加并达高峰(P<0.05),以后逐渐下降并接近正常。创伤组各时段TIMP-1蛋白表达与对照组相比,差别无统计学意义(P>0.05)。结论大鼠肾脏机械性损伤后MMP-9是参与肾脏损伤的重要因素之一。Objective To investigate the expressions and significance of matrix metalloprotease-9 （MMP-9） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in kidneys of rats after mechanical injury. Methods Thirty-six adult female Wistar rats were randomly divided into two groups： 6 in control group and 30 in trauma group. The model of trauma rats was constructed by striking the spinal column and ribs of rats with free-falling bio-impactor. MMP-9 and TIMP-I expressions in renal tissues of each group were detected by tissue chips and immunohistochemistry at 1, 6, 12, 24 and 86 h after trauma, Results MMP-9 group while it began to increase at 1 h, reached expression of renal tissues was lower in control high peak at 6 h （P 〈0.05） and then dropped gradually to normal in trauma group. TIMP-1 remained relatively unaltered in trauma group Conclusion MMP-9 may play an important role in kidney injury after mechanical injury.

关 键 词：肾 创伤 基质金属蛋白酶9 组织金属蛋白酶抑制剂1 组织芯片 

分 类 号：R641[医药卫生—外科学]