高效液相色谱法测定消癌平颗粒剂中绿原酸的含量  

Determination of Chlorogenic acid in Xiaoaiping particle by HPLC

作　　者：李瑞麟[1] 范鲁雁[1] 刘骅[2] 

机构地区：[1]安徽医科大学第三附属医院.合肥市第一人民医院药学部,安徽合肥230061 [2]安徽省食品药品检验所,安徽合肥230051

出　　处：《安徽医药》2008年第12期1163-1164,共2页Anhui Medical and Pharmaceutical Journal

基　　金：安徽省第三周期临床医学特色专科专项基金资助[(省卫科秘2007(765)号]

摘　　要：目的探讨消癌平颗粒剂中绿原酸含量测定的HPLC法。方法采用HPLC法,色谱条件：LUNA ODSC18柱;以乙腈-0.4%磷酸溶液（9∶91）为流动相;流速为1.0 ml.min^-1,检测波长327 nm,柱温25℃。结果0.015-0.600μg范围内呈良好的线性关系r=0.9999;平均回收率为99.00%,RSD为0.83%（n=5）。结论本方法快速、简便,结果准确、可靠,可用于消癌平颗粒中绿原酸的含量测定。Aim To establish a method for the determination of chlorogenic acid in Xiaoaiping particle. Methods Chlorogenic acid was chromatographed on Shimadzu HPLC with a C18 column and detected at 327nm. The mobile phase was acetonitrile-0.4% phosphate acid （9：91 ）. The flow rate was 1.0 ml · min^-1. The column temperature was 25 ℃. Results The linear range of the method was between 0. 015 and 0. 600 ug （ r = 0.999 9 ）. The average recovery was 99.00% , and RSD was 0.83 % （ n = 5 ）. Conclusion The method is simple, fast and accurate for the analysis of chlorogenic acid.

关 键 词：消癌平颗粒 绿原酸 高效液相色谱法 HPLC 测定 

分 类 号：R286.0[医药卫生—中药学]