川芎嗪对早期糖尿病大鼠股骨头组织中醛糖还原酶、一氧化氮、一氧化氮酶及钠-钾-ATP酶的影响  

Influence of ligustrazine on aldose reductase,nitric oxide,nitric oxide synthase and Na-K-ATP enzyme in the femoral head tissue of rats with early diabetes

作　　者：陈小萍[1] 陈海英[2] 陈小兰[1] 

机构地区：[1]莆田学院基础医学院生物化学教研室,福建莆田351100 [2]莆田学院基础医学院组织学与胚胎学教研室,福建莆田351100

出　　处：《新乡医学院学报》2008年第6期553-555,共3页Journal of Xinxiang Medical University

基　　金：福建省教育厅自然科学基金资助项目(编号:JA08189)

摘　　要：目的探讨川芎嗪对早期糖尿病(DM)大鼠股骨头中醛糖还原酶(AR)、一氧化氮(NO)、一氧化氮酶(NOS)系统及钠-钾-ATP酶(Na+-K+-ATPase)的影响。方法选择SD大鼠,随机分为对照组、DM模型组和川芎嗪组。一次性腹腔注射链脲佐菌素(STZ)60 mg.kg-1诱发糖尿病,分别于30 d和60 d测定各组大鼠股骨头匀浆中的NO含量、NOS活性、Na+-K+-ATPase及AR含量。结果30 d、60 d时DM组的NO、NOS和Na+-K+-ATPase均低于对照组和川芎嗪组(P<0.01),AR均高于对照组和川芎嗪组(P<0.01);30 d时川芎嗪组NO、NOS、AR和Na+-K+-ATPase与对照组比较无明显差异(P>0.05);60 d时川芎嗪组NO、NOS和Na+-K+-ATPase均低于对照组(P<0.01),AR高于川芎嗪组(P<0.01);60 d时DM组和川芎嗪组大鼠股骨头匀浆中NO含量和NOS活性和Na+-K+-ATPase均明显低于30 d时(P<0.01),AR均高于30 d时(P<0.01)。结论川芎嗪能逆转股骨头组织中AR、NO、NOS及Na+-K+-AT-Pase活性,对防治早期DM大鼠股骨头病变有一定的效果。Objective Influence of ligustrazine on aldose reductase（AR） ,nitric oxide（NO）,nitric oxide synthase（NOS） and Na-K-ATP enzyme（ Na^＋ -K^＋ -ATPase） in the femoral head tissue of rats with early diabetes. Methods Sixty SD rats were randomly divided into control group,diabetes mellitus（DM） group and ligustrazine group. Diabetes medal was induced by 60 mg · kg^-1 of streptozotocin（STZ） with one-time intraperitoneal injection. The content of NO,AR, Na^＋ -K ^＋ -ATPase and the activity of NOS in homogenates were detected at thirty days and sixty days in every group. Results The content of NO,NOS and Na^＋ -K^＋ -ATPase in DM group were lower than these in control group and treatment group at thirty days and sixty days（ P 〈 0.01 ） ,and the level of AR was higher than normal group and ligustrazine group（P 〈 0.01 ） ;There was no significant difference in the level of NO, NOS, AR and Na^＋ -K^＋ -ATPase between lignstrazine group and control group at thirty days （ P 〉 0.05 ） ; The level of NO, NOS and Na^＋ -K^＋ -ATPase in ligustrazine group were lower than these in control group（ P 〈 0.01 ）, and the level of AR was higher than this in control group at sixty days（ P 〈 0.01 ） ;The levels of NO, NOS and Na^＋ -K^＋ -ATPase in DM group and ligustrazine group at sixty days were significantly lower than these at thirty days（ P 〈 0.01 ）, and the level of AR was higher than at thirty days（ P 〈 0.01 ）. Conclusion Lignstrazin can reverse the level of AR, NO, NOS and Na^＋ -K^＋ -ATPase in the femoral head, so there are certain effect on prevention and treatment of early lesion of the femoral head in DM rats.

关 键 词：醛糖还原酶 川芎嗪 糖尿病 链脲菌素 

分 类 号：R965[医药卫生—药理学]