测定巨细胞病毒特异性抗体亲和指数的诊断价值  

Value of determination of specific cytomegalovirus-IgG (CMV-IgG) antibody′s avidity index in the diagnostic of CMV primary infection

作　　者：牛向兰 侯林浦[2] 李欣[2] 谷学英 马旭[2] 

机构地区：[1]深圳市人口和计划生育科学研究所优生遗传实验中心,广东518048 [2]国家人口计生委科学技术研究所优生遗传室

出　　处：《中国生育健康杂志》2008年第5期293-296,共4页Chinese Journal of Reproductive Health

摘　　要：目的以快速、简便的人巨细胞病毒特异性抗体亲和指数(CMV-IgG AI)检测方法作为有效的诊断工具,用于区分孕妇CMV原发感染与非原发感染。方法以标准的间接ELISA方法,8.0 mol/L尿素作为温和蛋白变性剂,用标准曲线方法定量测定标本中的CMV-IgG含量。结果共检测218例标本,其中187例为CMV既往感染标本,31例为继发或再感染、可疑原发感染和原发感染标本。以抗体亲和指数<35.0%作为阳性判断标准,此时方法的灵敏度为75.0%,特异度为91.4%,可用度为67.2%。既往感染、继发或再感染、可疑原发感染、原发感染4组的平均AI值分别是(61.42±25.69)%,(70.38±21.88)%,(66.73±23.62)%,(29.78±25.93)%,原发感染组与其它3组非原发感染比较,差异有统计学意义(P<0.01))。结论以8.0 mol/L尿素作为温和蛋白变性剂,用标准曲线方法定量测定标本中CMV-IgG含量适用于检测巨细胞病毒IgG抗体亲和指数(CMV-IgG AI),认为CMV-IgM联合CMV-IgG AI检测是区分原发与非原发感染的血清学方法之一。Objective To establish a rapid, easy and simple method for determination of CMV - IgG AI to serve as effective diagnostic tool to distinguish primary CMV infection from non - primary infection in pregnant women. Methods The measurement of CMV - IgG AI is accomplished by standard indirect ELISA method using 8 mol/L urea as a mild protein denaturing agent and the amount of CMV - IgG in the specimen was measured quantitatively using a standard curve method. Results Of the 218 samples, 187 were from women with past CMV infection and 31 from those with secondary infection or re -infection, suspicious primary infection and primary infection. When the CMV - IgG AI less than 35.0 % was defined as positive, the sensitivity was 75. 0 %, the specificity was 91.4 %, and availability was 67. 2 %. The mean AI in the samples from women with past infection , secondary infection or re - infection, suspicious primary infection and primary infection was （ 61.42 ± 25.69 ） %, （ 70. 38 ± 21.88 ） %, （ 66. 73 ± 23.62 ） %, （29. 78 ± 25.93 ） %, respectively. There is a statistical difference between the primary and non - primary CMV infection （ P 〈 0. 01 ）. Conclusion The amount of CMV - IgG in the specimen quantitatively measured by a standard curve with 8 m urea solution as a mild protein denaturing agent, was found to be suitable for determination CMV - IgG AI. At present the combination of CMV - IgG AI and CMV - IgM assay seem to be one of the best serological tests for differentiating primary CMV infection from non - primary CMV infection.

关 键 词：巨细胞病毒 亲和指数 IGG抗体 原发感染 酶联免疫吸附试验 

分 类 号：R446.6[医药卫生—诊断学]