亚心形四爿藻培养和产氢过程一体化平板光生物反应系统  

Integrated Cultivation and Photohydrogen Production of Tetraselmis Subcordiformis in a Flat-plate Photobioreactor System

作　　者：郑阳 陈兆安[1] 傅赟彬[1,2] 陆洪斌[1] 张卫[1] 

机构地区：[1]中国科学院大连化学物理研究所海洋产品工程组,大连116023 [2]中国科学院研究生院,北京100049

出　　处：《高等学校化学学报》2008年第11期2209-2212,共4页Chemical Journal of Chinese Universities

基　　金：国家“九七三”计划(批准号:2003CB214500);国家“八六三”计划(批准号:2006AA05Z106);中国科学院知识创新工程重要方向项目基金(批准号:KSCX2-YW-G-002);中国科学院知识创新工程领域前沿项目基金资助

摘　　要：利用整合了燃料电池的平板光生物反应器,探讨了将亚心型四爿藻高密度培养和产氢两段工艺一体化集成的可行性.在培养阶段通入体积分数为2%～5%的CO2可使藻细胞迅速增殖,9 d内即可达到产氢要求的生物量（8.5×10^6cell/mL）.通过叶绿素荧光参数分析,选择2%的CO2培养的藻进行后续的产氢实验.结果表明,PSⅡ活性和光合电子传递速率均随时间的推移而逐渐下降.通过对产氢动力学曲线的分析,计算出最大产氢速率为1.1 mL/（h·L）,持续产氢时间为60 h.This paper aimed to develop an integrated processes of microalgal cultivation and hydrogen photoproduction in a fiat-plate photobioreactor system combined with alkaline fuel cell. The optimal high cell density of 8.5 × 10^6 cell/mL was achieved on 9 d when the cultivation of Tetraselmis subcordiformis was supplemented with CO2 of 2%-5% （volume fraction） in the flat-plate photobioreactor. When the bioreactor system was switched to the hydrogen production condition, hydrogen evolution was induced by the addition of carbonylcyanide （m-chlorophenylhydrazone, CCCP） and anaerobic condition, with a maximum hydrogen production rate of 1.1 mL/（ h · L） for 60 h. The kinetics analysis of algal chlorophyll fluorescence was carried out to identify the limiting factors in both algal growth and hydrogen production at 2% CO2 supplementation. At hydrogen production phase, algal PS 11 activity and relative electron transfer rate （rETR） decreased with the increase of time, which caused the decline and eventual stop in hydrogen production. To sustain the hydrogen production, PS Ⅱ activity and rETR must be maintained. The results demonstrate the feasibility of process integration of algal cultivation and hydrogen production in one single system, which will significantly improve the process economies.

关 键 词：平板光生物反应器 一体化工艺 亚心形四爿藻(Tetraselmis subcordiformis) 叶绿素荧光 燃料电池 

分 类 号：Q949.2[生物学—植物学]