Vero细胞形变表征污染物生物毒性的可靠性分析  

Reliability of Morphological Changes of Vero Cells for Indication of Biotoxicity of Pollutants

作　　者：贾建伟[1] 石艳玲[1] 王磊[1] 

机构地区：[1]同济大学污染控制与资源化研究国家重点实验室,上海200092

出　　处：《中国给水排水》2008年第23期89-91,94,共4页China Water & Wastewater

基　　金：国家自然科学基金资助项目(20677043);上海市自然科学基金资助项目(05ZR14119)

摘　　要：考察了2,4,6-三氯苯酚(TCP)和饮用水相关材料(PVC管和一次性塑料水杯)的浸泡液致Vero细胞变形的灵敏度与相关性,并与四氮甲唑蓝(MTT)比色法进行了比较。结果表明:TCP对Vero细胞的毒性响应浓度为0.5mg/L,且变形细胞的比例与TCP浓度呈正相关,而MTT比色法测定低浓度TCP(<1.0mg/L)致Vero细胞增殖抑制率的结果不稳定;流式细胞仪AN-NEXINV-FITC/PI双染色法的检测结果显示,细胞膜损伤是Vero细胞毒性的早期表现特征,比细胞凋亡和坏死更灵敏;Vero细胞暴露于饮用水相关材料的浸泡液时,其变形细胞的比例随浸泡温度的升高而增加,而在相同条件下,MTT比色法测定的浸泡液对Vero细胞的增殖抑制率则呈现不规律变化。The morphological changes of Vero cells exposed to 2, 4, soaking solutions of the products related to drinking water （ PVC pipe and 6-trichlorophenol （TCP） and disposable plastic cup） were studied, and their sensitivity and correlation were compared to that of MTT assay. The results indicate that the toxicity response concentration of TCP for Vero cells is 0.5 mg/L, and the proportion of morphologically changed cells is positively correlative to the TCP concentration obviously. However, under the low TCP concentration （less than 1.0 mg/L）, the inhibition of cell viability tested by MTT assay seems to be irregular. The analysis results of flow cytometry with ANNEXIN V-FITC/PI double staining method show that cell membrane damage is the early cytotoxic response prior to the apoptosis and necrosis. Exposed to the soak solutions at different temperature, the percentage of morphologically c hanged cells increases with rise of soaking temperature, but MTT assay fails to reflect the correlation between soaking temperature and viability inhibition of Vero cells.

关 键 词：VERO细胞 2 4 6-三氯苯酚 形态变化 污染物 生物毒性 水质 水厂 MTT比色法 

分 类 号：TU991.2[建筑科学—市政工程]