拉曼光谱研究复方鹿仙草颗粒对SMMC-7721肝癌细胞的作用  

Raman Spectroscopic Investigation on the Interactions between Liver Cancer Cells(SMMC-7721) and Fufang Luxiancao Particles

作　　者：张金彦[1] 郭建宇[1] 蔡炜颖[1] 孙真荣[1] 

机构地区：[1]精密光谱科学与技术国家重点实验室(华东师范大学),华东师范大学物理系,上海200062

出　　处：《光谱学与光谱分析》2008年第11期2574-2578,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(10574046);国家基础研究重大项目(2006CB806006,2006CB921105);教育部新世纪人才计划项目(NCET-04-0420);高等学校博士点基金项目(20050269011);上海市科委科技启明星计划项目(06QH14003)资助

摘　　要：应用拉曼光谱研究了不同浓度的鹿仙草溶液与肝癌细胞SMMC-7721的相互作用,通过对药物作用前后细胞的光谱变化进行分析,从而为阐明鹿仙草与肝癌细胞的作用方式提供重要的依据。拉曼光谱显示,加入鹿仙草后,细胞的许多峰都发生了变化。归属于磷酸骨架振动的785和1 092 cm-1的两个峰强度下降,对应碱基A和G的峰1 312和1 585 cm-1等也有不同程度的降低,表明鹿仙草可能插入DNA碱基对之间,使DNA复制受到抑制,导致细胞DNA含量下降,而且会引起DNA单、双链的断裂。同时,研究还发现属于蛋白质的振动峰(1 005,1 360,1 656 cm-1)强度也有不同程度下降,说明蛋白质二级结构以及侧链氨基酸的环境均发生了改变。此外,鹿仙草对细胞的作用效果随浓度增加逐步增强。Luxiancao is a new medicine for liver cancer,and is purely natural botanical.It has good curative effects and few side effects.The curative mechanism of Luxiancao is unknown.In the present paper,the authors used a 514.5 nm laser to measure the changes in the Raman spectrum of liver cancer cells（SMMC-7721） treated by Luxiancao at different concentrations.The study can help us know more about the mechanism,efficiency and side effects of Luxiancao. The results show that significant changes were observed in the cells＇ Raman spectra after reacting with Luxiancao.The intensities at 785 and 1 092 cm-1, corresponding to DNA phosphate backbone vibration,were reduced,and the Raman bands for the bases A and G at 1 312 and 1 585 cm-1 also decreased,indicating that Luxiancao may be inserted in DNA bases and influence the DNA replications,resulting in the reduction in DNA content and breaking of the DNA strands.Besides,the intensity of 1 360 cm-1,belonging to Trp,decreased gradually and disappeared in the end,indicating the Trp of cancer cells began to be exposed when adding in Luxiancao.The bands at 1 004 cm-1 for Phe and 1 656 cm-1 for proteins α-helix also decreased,suggesting that there were changes in the structure of protein and circumstance of amino acid.Moreover,the effects on cancer cells were enhanced gradually with the HCPT concentration increasing.Since a Raman spectrum is a chemical fingerprint of a sample,the different concentration dependent changes in the Raman spectra of individual cells due to reacting with Luxiancao can overcome the limitations of other detection systems used for quantitative and qualitative analysis of the drug.

关 键 词：拉曼光谱 鹿仙草 肝癌细胞 

分 类 号：O657.3[理学—分析化学]