外源性IL-10对单核细胞定向分化为树突状细胞的影响  

Effect of IL-10 on the differentiation of monocyte-derived dendritic cells

作　　者：姜朋涛[1] 邢美芬[1] 张戎[1] 孙志达[1] 杨晓帆[1] 王慧娟[1] 顾镭[2] 张缪佳[2] 季晓辉[1] 

机构地区：[1]南京医科大学基础医学院微生物与免疫学系,江苏南京210029 [2]南京医科大学第一附属医院风湿科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2008年第11期1398-1401,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省自然科学基金资助(BK2004148)

摘　　要：目的:研究外源性IL-10对单核来源的树突状细胞(dendritic cells,DCs)的影响,为进一步认识IL-10在系统性红斑狼疮(system lupus erythemacosus,SLE)发病机制中的作用提供线索。方法:应用GM-CSF+IL-4+TNF-α体系诱导培养单核细胞来源的DCs,同时在该体系中加入与SLE患者血清浓度相当的外源性IL-10(30 pg/ml)。用流式细胞术检测DCs的表型(CD14、CD11c、CD1a、HLA-DR、CD80、CD86和CD83),CCK-8法检测Dcs刺激同种异体淋巴细胞增殖能力,ELISA法检测DCs分泌IL-12p40、IL-10和INF-γ水平。结果:30 pg/ml的外源性IL-10可使单核细胞来源的DCs(monocyte derived-DCs,MDDCs)HLA-DR、CD80、CD86和CD83的表达以及IL-12p40和IFN-γ的分泌水平受到抑制,并使其刺激同种异体T细胞增殖能力下降。结论:外源性IL-10对单核细胞定向分化为DCs产生了抑制作用。进一步证实IL-10在SLE发病机制中发挥了重要作用。Objective：To further investigate the realation between IL-10 and the pathogeneSis of SLE. Methods：MDDCs was cultured with 30 pg/ml IL-10 and 0 pg/ml IL-10. The phenotypes of these DCs（CD14,CDllc,CDla,HLA-DR,CD80,CD86 and CD83） were analyzed by flow eytometer. The stimulating altogenic lymphocytes proliferation abilities of these DCs were checked by CCK-8. ELISA was used to measure IL-12p40,IL-10 and INF-γ concentrations of the MDDCs culture supematants. Results：The expression of HLA-DR, CD86,CD80 and CD83 of MDDCs cultured with 30 pg/ml IL-10 was lower than that of MDDCs without IL-10. MDDCs treated with 30 pg/ml IL-10 showed decreased abilities of stimulating allogenic T cells proliferation. The IL-12p40 ,IL-10 and INF-γ coneentrations were no significant alteration. Conclusion： Exogenous IL-10 inhibited the differentiation and function of MDDCs. These results further showed that IL-10 plays an important role in the pathogenesis of SLE.

关 键 词：红斑狼疮 系统性 IL-10 树突状细胞 单核细胞 

分 类 号：R593.241[医药卫生—内科学]