复黄片微生物限度检查法的建立  

Establishment of microbial limit test for Fuhuang tablets

作　　者：李继扬[1] 陆金根[2] 曹永清[2] 邱明丰[3] 

机构地区：[1]复旦大学药学院生物合成教研室,上海200032 [2]上海中医药大学附属龙华医院肛肠科,上海200032 [3]上海交通大学药学院药剂教研室,上海200240

出　　处：《复旦学报（医学版）》2008年第6期922-924,934,共4页Fudan University Journal of Medical Sciences

基　　金：上海市重点学科建设项目(T0304)

摘　　要：目的建立复黄片微生物限度检查法。方法通过培养基稀释法、菌体离心沉淀法等测定复黄片对试验菌株的回收率。结果复黄片对金黄色葡萄球菌和枯草芽孢杆菌及白色念珠菌、黑根霉呈现较强的抑菌作用,而对大肠埃希菌基本上没有抑制作用。采用离心沉淀法可以消除复黄片对待测微生物的影响。结论复黄片可用离心沉淀法进行微生物限度的检查。Objective To establish microbial limit test （MLT） for Fuhuang tablets. Methods With centrifugal sedmentation and culture medium diluted methods, the recovery test was done on each bacterial strain to validate the accuracy and feasibility of MLT. Results Fuhuang tablets showed a stronger inhibitory effect on Staphylococcus aureus, Bacillus subtilis, Candida albicans and Aspergillus niger, had no inhibitory effect on Escherichia coll. Conclusions Centrifugal sedmentation method can eliminate the antibacterial effect of Fuhuang tablets during the experiment, thus the microorganism in the polluted sample can be detected successfully.

关 键 词：复黄片 微生物限度检查 方法验证 

分 类 号：R286.02[医药卫生—中药学]